Source:http://linkedlifedata.com/resource/pubmed/id/18726586
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2008-10-21
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pubmed:abstractText |
In hygiene management, recently there has been a significant need for screening methods for microbial contamination by visual observation or with commonly used colorimetric apparatus. The amount of adenosine triphosphate (ATP) can serve as the index of a microorganism. This paper describes the development of a colorimetric method for the assay of ATP, using enzymatic cycling and Fe(III)-xylenol orange (XO) complex formation. The color characteristics of the Fe(III)-XO complexes, which show a distinct color change from yellow to purple, assist the visual observation in screening work. In this method, a trace amount of ATP was converted to pyruvate, which was further amplified exponentially with coupled enzymatic reactions. Eventually, pyruvate was converted to the Fe(III)-XO complexes through pyruvate oxidase reaction and Fe(II) oxidation. As the assay result, yellow or purple color was observed: A yellow color indicates that the ATP concentration is lower than the criterion of the test, and a purple color indicates that the ATP concentration is higher than the criterion. The method was applied to the assay of ATP extracted from Escherichia coli cells added to cow milk.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Ferric Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Ferrous Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Organometallic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvate Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Xylenes,
http://linkedlifedata.com/resource/pubmed/chemical/xylenol orange
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1618-2650
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
392
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
987-94
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pubmed:meshHeading |
pubmed-meshheading:18726586-Adenosine Triphosphate,
pubmed-meshheading:18726586-Animals,
pubmed-meshheading:18726586-Biological Assay,
pubmed-meshheading:18726586-Cattle,
pubmed-meshheading:18726586-Cell Line,
pubmed-meshheading:18726586-Colorimetry,
pubmed-meshheading:18726586-Escherichia coli,
pubmed-meshheading:18726586-Ferric Compounds,
pubmed-meshheading:18726586-Ferrous Compounds,
pubmed-meshheading:18726586-Milk,
pubmed-meshheading:18726586-Organometallic Compounds,
pubmed-meshheading:18726586-Oxidation-Reduction,
pubmed-meshheading:18726586-Pyruvate Oxidase,
pubmed-meshheading:18726586-Xylenes
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pubmed:year |
2008
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pubmed:articleTitle |
Colorimetric method for enzymatic screening assay of ATP using Fe(III)-xylenol orange complex formation.
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pubmed:affiliation |
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Nishi 8, Kita 13, Sapporo, 060-8628, Japan. ishida-a@eng.hokudai.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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