Linked Life Data

18726335

Source:http://linkedlifedata.com/resource/pubmed/id/18726335

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-8-26
pubmed:abstractText
By using DNA-specific fluorescent dye and a confocal laser scanning microscope, the present study was designed to investigate the cytological characteristics of dedifferentiating initiation during pretreatment and embryogenesis during culture in freshly-isolated microspores of barley, and the difference in main developmental pathway between freshly-isolated and cold-treated microspores. The results revealed that (i) freshly-isolated microspores started the initiation within 12 h of mannitol pretreatment, whose main cytological characteristics were that: cell volume was obviously extended; the volume of nuclei and nucleoli were also greatly increased; nucleoli were extremely clear and highly condensed; N/C ratio was very high; (ii) all the pretreatment methods led to the initiation of the microspores, thus triggering the embryogenic process; (iii) pretreatment methods influenced the main developmental pathway of microspores by changing the pattern of the first mitosis. The cold-treated microspores formed main developmental pathway via A patterns, but freshly-isolated microspores via B pattern.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jun
pubmed:issn
1006-9305
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
332-6
pubmed:year
1997
pubmed:articleTitle
Dedifferentiating initiation and embryogenesis from freshly-isolated microspores of barley.
pubmed:affiliation
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics, Chinese Academy of Sciences, 100101, Beijing, China.
pubmed:publicationType
Journal Article