Linked Life Data

18722167

Source:http://linkedlifedata.com/resource/pubmed/id/18722167

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-11-26
pubmed:abstractText
Ca-deficient hydroxyapatite (CDHA) porous scaffolds were successfully fabricated from calcium phosphate cement (CPC) by a particle-leaching method. The morphology, porosity and mechanical strength as well as degradation of the scaffolds were characterized. The results showed that the CDHA scaffolds with a porosity of 81% showed open macropores with pore sizes of 400-500mum. Thirty-six per cent of these CDHA scaffolds were degraded after 12 weeks in Tris-HCl solution. Mesenchymal stem cells (MSCs) were cultured, expanded and seeded on the scaffolds, and the proliferation and differentiation of MSCs into osteoblastic phenotype were determined using MTT assay, alkaline phosphatase activity and scanning electron microscopy. The results revealed that the CDHA scaffolds were biocompatible and had no negative effects on the MSCs in vitro. The in vivo biocompatibility and osteogenicity of the scaffolds were investigated. Both CDHA scaffolds and MSC/scaffold constructs were implanted in rabbit mandibles and studied histologically. The results showed that CDHA scaffolds exhibited good biocompatibility and osteoconductivity. Moreover, the introduction of MSCs into the scaffolds dramatically enhanced the efficiency of new bone formation, especially at the initial stage after implantation (from 2 to 4 weeks). However, the CDHA scaffolds showed as good biocompatibility and osteogenicity as the hybrid ones at 8 weeks. These results indicate that the CDHA scaffolds fulfill the basic requirements of bone tissue engineering scaffold.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1878-7568
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
268-78
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering.
pubmed:affiliation
Key Laboratory for Ultrafine Materials of Ministry of Education, and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't