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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2008-10-21
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pubmed:abstractText |
Teicoplanin (teic) from Actinoplanes teichomyceticus is a glycopeptide antibiotic used to treat many gram-positive bacterial infections. Glycopeptide antibiotics inhibit bacterial growth by binding to carboxy-terminal D-Ala-D-Ala intermediates in the peptidoglycan of the cell wall of gram-positive bacteria. In this paper we report the derivatization of magnetic microspheres with teic (teic-microspheres). Fluorescence-based techniques have been developed to analyze the binding properties of the microspheres to two D-Ala-D-Ala terminus peptides. The dissociation constant for the binding of carboxyfluorescein-labeled D-Ala-D-Ala-D-Ala to teic on microspheres was established via fluorimetry and flow cytometry and was determined to be 0.5 x 10(-6) and 3.0 x 10(-6) mol L(-1), respectively. The feasibility of utilizing microparticles with fluorescence methods to detect low levels (the limit of bacterial detection was determined to be 30 colon-forming units; cfu) of gram-positive bacteria has been demonstrated. A simple microfluidic experiment is reported to demonstrate the possibility of developing microsphere-based affinity assays to study peptide-antibiotic interaction.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-10565276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-10579833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-10940250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-11791558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-12170179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-14677934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-15100857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-15700951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-15762582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-1645188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-649512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18712518-659325
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1618-2650
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
392
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
877-86
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pubmed:dateRevised |
2010-9-21
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pubmed:meshHeading |
pubmed-meshheading:18712518-Actinomycetales,
pubmed-meshheading:18712518-Anti-Bacterial Agents,
pubmed-meshheading:18712518-Bacteria,
pubmed-meshheading:18712518-Binding Sites,
pubmed-meshheading:18712518-Flow Cytometry,
pubmed-meshheading:18712518-Fluoresceins,
pubmed-meshheading:18712518-Fluorescence,
pubmed-meshheading:18712518-Magnetics,
pubmed-meshheading:18712518-Microfluidics,
pubmed-meshheading:18712518-Microscopy, Electron, Scanning,
pubmed-meshheading:18712518-Microspheres,
pubmed-meshheading:18712518-Peptides,
pubmed-meshheading:18712518-Staining and Labeling,
pubmed-meshheading:18712518-Teicoplanin,
pubmed-meshheading:18712518-Time Factors
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pubmed:year |
2008
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pubmed:articleTitle |
Magnetic microsphere-based methods to study the interaction of teicoplanin with peptides and bacteria.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032-8202, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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