Linked Life Data

1871140

Source:http://linkedlifedata.com/resource/pubmed/id/1871140

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1991-9-18
pubmed:abstractText
We have previously shown that the isometric tension of a fully calcium-activated skinned rabbit psoas muscle fiber is reversibly depressed by increased hydrostatic pressure. We report here the characterization of tension transients induced by a rapid (less than 1-ms) release of increased pressure at 12 degrees C. The tension transient consists of three clear phases, an initial further decrease of tension in phase with pressure change followed by two phases of tension increase back to the level recorded at ambient pressure. The mean reciprocal relaxation time for phase 2 (1/tau 2) was approximately 17 s-1 and that for phase 3 (1/tau 3) was 3 s-1. The presence of 20 mM inorganic phosphate markedly increased 1/tau 2 to approximately 52 s-1 and decreased 1/tau 3 to approximately 1.7 s-1. These observations are interpreted in terms of a pressure-sensitive transition between two attached crossbridge states of low (or zero) and higher force. This is compatible with the pressure-sensitive isomerization of actomyosin previously observed in solution. The results presented allow us to propose a coupling between a specific pressure-sensitive isomerization of purified actomyosin, the phosphate release step of the ATPase pathway, and the force-generating event of the cross-bridge cycle.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-12793, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-1825780, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-2383626, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-2527246, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-2605297, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-2760190, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-2892206, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-2946675, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-302333, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-3156404, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-3159090, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-3162018, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-3350808, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-3446791, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-3469654, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-3878160, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-4091793, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-494240, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-4960130, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-6237117, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-6611412, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-6973625, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-6996582, http://linkedlifedata.com/resource/pubmed/commentcorrection/1871140-7198909
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7323-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Tension responses to rapid pressure release in glycerinated rabbit muscle fibers.
pubmed:affiliation
Department of Biochemistry, University of Bristol, School of Medical Sciences, United Kingdom.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't