Linked Life Data

18708067

Source:http://linkedlifedata.com/resource/pubmed/id/18708067

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-9-16
pubmed:abstractText
Aquaporin-4 (AQP4) water channels are expressed strongly in glial cells, where they play a role in brain water balance, neuroexcitation, and glial cell migration. Here, we investigated a proposed new role of AQP4 in facilitating cell-cell adhesion. Measurements were made in differentiated primary glial cell cultures from wild-type versus AQP4 knockout mice as well as in null versus AQP4-transfected L-cells, a cell type lacking endogenous adhesion molecules, and in null versus AQP4-transfected Chinese hamster ovary (CHO)-K1 cells and Fisher rat thyroid cells. Using established assays of cell-cell adhesion, we found no significant effect of AQP4 expression on adhesion in each of the cell types. As a positive control, transfection with E-cadherin greatly increased cell-cell adhesion. High-level AQP4 expression also did not affect aggregation of plasma membrane vesicles in a sensitive quasi-elastic light-scattering assay. Further, we found no specific AQP4 binding of a fluorescently labeled oligopeptide containing the putative adhesion sequence in the second extracellular loop of AQP4. These data provide evidence against involvement of AQP4 in cell-cell adhesion.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1089-8638
pubmed:author
pubmed:issnType
Electronic
pubmed:day
24
pubmed:volume
382
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1136-43
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Evidence against involvement of aquaporin-4 in cell-cell adhesion.
pubmed:affiliation
Department of Medicine, University of California, 1246 Health Sciences East Tower, San Francisco, CA 94143-0521, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural