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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1991-9-19
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pubmed:abstractText |
Turnip crinkle virus is one of several single-stranded RNA plant viruses associated with defective interfering (DI) RNAs. A complete cDNA copy of a 344-base DI RNA (DI RNA G) was cloned downstream from a T7 RNA polymerase promoter. Transcripts synthesized in vitro were infectious when inoculated with helper virus on turnip plants. Studies of the infectivity of DI transcripts containing deletions, insertions, and single-base changes suggest that (i) in general, only the 5' two-thirds of the molecule can tolerate mutations; (ii) between 52 and 67 bases of terminal 5' sequence are required for infectivity; (iii) nucleotides in positions 68 to 138 are not specifically involved in RNA infectivity; (iv) DI RNA G molecules smaller than 327 bases are not amplified efficiently in plants.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-16453740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-1693330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-1697328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-1957718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-2005430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-2243386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-2370670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-2594759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-2718380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-2718381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-3047690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-3181135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-3664641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-3753584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-3811230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-5439728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-6259811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1870188-7026180
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4582-90
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:1870188-Base Sequence,
pubmed-meshheading:1870188-Chromosome Deletion,
pubmed-meshheading:1870188-Cloning, Molecular,
pubmed-meshheading:1870188-DNA Mutational Analysis,
pubmed-meshheading:1870188-Defective Viruses,
pubmed-meshheading:1870188-Molecular Sequence Data,
pubmed-meshheading:1870188-Plant Viruses,
pubmed-meshheading:1870188-RNA, Viral,
pubmed-meshheading:1870188-Restriction Mapping,
pubmed-meshheading:1870188-Vegetables,
pubmed-meshheading:1870188-Viral Interference,
pubmed-meshheading:1870188-Virus Replication
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pubmed:year |
1991
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pubmed:articleTitle |
In vivo accumulation of a turnip crinkle virus defective interfering RNA is affected by alterations in size and sequence.
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pubmed:affiliation |
Department of Plant Pathology, University of Massachusetts, Amherst 01003.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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