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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2008-10-20
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pubmed:abstractText |
A sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed to investigate isosteviol pharmacokinetics in vivo. Isosteviol was extracted from plasma with hexane and 4% formic acid. A Phenomenex Synergi 2mu Fusion reversed phase analytical HPLC column (50 mm x 2.0 mm) equipped with a Synergi 2micro Fusion guard column was employed for chromatographic separations. The gradient mobile phase consisted of acetonitrile (ACN) and 20mM ammonium acetate at pH 6.5, starting at 20% ACN and ramping to 80% at 7 min, followed by 80% ACN for 1 min, then 20% ACN for 5 min. Negative SRM was used to monitor the m/z 317.1/317.1 and 317.3/317.3 transitions for isosteviol and 395.0/395.0 and 397.0/397.0 transitions for internal standard. The retention time of isosteviol was 9.2 min. The assay was linear over the range of 50-2,000 ng/mL. The accuracy of the method was in the range of 97-105%. Intra- and inter-day precisions were in the range of 1.5-4.6%. Isosteviol (4 mg/kg) was dosed intravenously and orally to Sprague-Dawley rats (n=6). Plasma samples were collected and analysed. Intravenous isosteviol has a distribution half-life of 35.7+/-9.0 min with the initial distribution volume of 68.1+/-9.4 mL. The total clearance, terminal half-life and steady-state volume of distribution were 1.25+/-0.12 mL/min, 150.6+/-50.5 min and 272.6+/-95.9 mL, respectively. The oral bioavailability of isosteviol was found to be 60.4+/-15.5%.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0731-7085
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
986-90
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pubmed:meshHeading |
pubmed-meshheading:18701231-Acetates,
pubmed-meshheading:18701231-Acetonitriles,
pubmed-meshheading:18701231-Administration, Oral,
pubmed-meshheading:18701231-Animals,
pubmed-meshheading:18701231-Area Under Curve,
pubmed-meshheading:18701231-Biological Availability,
pubmed-meshheading:18701231-Calibration,
pubmed-meshheading:18701231-Chromatography, Liquid,
pubmed-meshheading:18701231-Diterpenes, Kaurane,
pubmed-meshheading:18701231-Drug Stability,
pubmed-meshheading:18701231-Freezing,
pubmed-meshheading:18701231-Half-Life,
pubmed-meshheading:18701231-Hydrogen-Ion Concentration,
pubmed-meshheading:18701231-Injections, Intravenous,
pubmed-meshheading:18701231-Male,
pubmed-meshheading:18701231-Metabolic Clearance Rate,
pubmed-meshheading:18701231-Molecular Structure,
pubmed-meshheading:18701231-Rats,
pubmed-meshheading:18701231-Rats, Sprague-Dawley,
pubmed-meshheading:18701231-Reference Standards,
pubmed-meshheading:18701231-Reproducibility of Results,
pubmed-meshheading:18701231-Sensitivity and Specificity,
pubmed-meshheading:18701231-Solvents,
pubmed-meshheading:18701231-Sweetening Agents,
pubmed-meshheading:18701231-Tandem Mass Spectrometry,
pubmed-meshheading:18701231-Temperature
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pubmed:year |
2008
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pubmed:articleTitle |
Oral and i.v. pharmacokinetics of isosteviol in rats as assessed by a new sensitive LC-MS/MS method.
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pubmed:affiliation |
Sansom Institute, City East, School of Pharmacy and Medical Science, University of South Australia, Adelaide, SA 5000, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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