Linked Life Data

18699781

Source:http://linkedlifedata.com/resource/pubmed/id/18699781

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2008-9-29
pubmed:abstractText
The ycaC-related gene, ycaCR, is uncharacterized, and has no assigned function to date. Here we clearly showed that the ycaC-related gene from the amphioxus Branchiostoma belcheri, BbycaCR, coded for a novel member of the isochorismatase superfamily, which is mainly localized in the mitochondrial fraction. Both pull-down and reverse pull-down analyses revealed that BbycaCR was able to interact with creatine kinase, an enzyme involved in energy transduction, in addition to binding to native ycaCR, forming a homopolymer. Surprisingly, neither isochorismatase, nicotinamidase nor N-carbamoylsarcosine amidohydrolase activity was detected for BbycaCR, although it possessed the putative catalytic triad of Asp19, Arg(Lys)84 and Cys118 that is found in ycaC proteins. Both tissue section in situ hybridization and immunohistochemistry showed that BbycaCR was ubiquitously expressed in amphioxus, although at different expression levels, suggesting that BbycaCR plays a conserved fundamental cellular role in amphioxus. It is proposed that BbycaCR may be indirectly involved in energy transduction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1742-464X
pubmed:author
pubmed:issnType
Print
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4597-605
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
The ycaC-related protein from the amphioxus Branchiostoma belcheri (BbycaCR) interacts with creatine kinase.
pubmed:affiliation
Department of Marine Biology, Ocean University of China, Qingdao, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't