Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-8-13
pubmed:abstractText
It has been estimated that up to 30% of detectable polyps in patients regress spontaneously. One major challenge in the evaluation of effective therapy of cancer is the readout for tumor regression and favorable biological response to therapy. Inducible near infra-red (NIR) fluorescent probes were utilized to visualize intestinal polyps of mice hemizygous for a novel truncation of the Adenomatous Polyposis coli (APC) gene. Laser Scanning Confocal Microscopy in live mice allowed visualization of cathepsin activity in richly vascularized benign dysplastic lesions. Using biotinylated suicide inhibitors we quantified increased activities of the Cathepsin B & Z in the polyps. More than (3/4) of the probe signal was localized in CD11b(+)Gr1(+) myeloid derived suppressor cells (MDSC) and CD11b(+)F4/80(+) macrophages infiltrating the lesions. Polyposis was attenuated through genetic ablation of cathepsin B, and suppressed by neutralization of TNFalpha in mice. In both cases, diminished probe signal was accounted for by loss of MDSC. Thus, in vivo NIR imaging of focal cathepsin activity reveals inflammatory reactions etiologically linked with cancer progression and is a suitable approach for monitoring response to therapy.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/R01 AI061701-01A1, http://linkedlifedata.com/resource/pubmed/grant/R01 AI061701-02, http://linkedlifedata.com/resource/pubmed/grant/R01 AI061701-03, http://linkedlifedata.com/resource/pubmed/grant/R01 AI061701-04, http://linkedlifedata.com/resource/pubmed/grant/R01 AI061701-05, http://linkedlifedata.com/resource/pubmed/grant/R01 CA104547-03, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-01, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-02, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-03, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-04, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-05, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-06, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-07, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-08, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-09, http://linkedlifedata.com/resource/pubmed/grant/R01 DK054778-10, http://linkedlifedata.com/resource/pubmed/grant/R01-CA125162
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-10625568, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-10668480, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-10987312, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-10995788, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-11385514, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-11832455, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-12186844, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-12612914, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-12965278, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-14717334, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-15144952, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-15652460, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-15852247, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-15899788, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-15967128, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16087401, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16331857, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16397525, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16481467, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16707449, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16732297, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16778222, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16885333, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-16943400, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-17184136, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-17245112, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-17360473, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-17524981, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-18077429, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-2296722, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-8631000, http://linkedlifedata.com/resource/pubmed/commentcorrection/18698347-9539769
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1932-6203
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
e2916
pubmed:dateRevised
2011-10-26
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Live imaging of cysteine-cathepsin activity reveals dynamics of focal inflammation, angiogenesis, and polyp growth.
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