Linked Life Data

18695940

Source:http://linkedlifedata.com/resource/pubmed/id/18695940

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2008-9-24
pubmed:abstractText
Human thyroid carcinoma XTC.UC1 cells harbor a homoplasmic frameshift mutation in the MT-ND1 subunit of respiratory complex I. When forced to use exclusively oxidative phosphorylation for energy production by inhibiting glycolysis, these cells triggered a caspase-independent cell death pathway, which was associated to a significant imbalance in glutathione homeostasis and a cleavage of the actin cytoskeleton. Overexpression of the anti-apoptotic Bcl-2 protein significantly increased the level of endogenous reduced glutathione, thus preventing its oxidation after the metabolic stress. Furthermore, Bcl-2 completely inhibited actin cleavage and increased cell adhesion, but was unable to improve cellular viability. Similar effects were obtained when XTC.UC1 cells were incubated with exogenous glutathione. We hence propose that Bcl-2 can safeguard cytoskeletal stability through an antioxidant function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1420-682X
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2943-51
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
The antioxidant function of Bcl-2 preserves cytoskeletal stability of cells with defective respiratory complex I.
pubmed:affiliation
Dipartimento di Biologia Evoluzionistica Sperimentale, Università di Bologna, Via Irnerio 42, Bologna 40122, Italy. annamaria.porcelli@unibo.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't