Linked Life Data

18695877

Source:http://linkedlifedata.com/resource/pubmed/id/18695877

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-8-12
pubmed:abstractText
Danshen (Salvia miltiorrhiza Bunge) is a herb that has been widely and successfully used for treating inflammatory diseases in clinics in Asia. The relatively abundant tanshinones, tanshinone I, tanshinone IIA, cryptotanshinone, and dihydrotanshinone, have been isolated from Danshen. These tanshinones are the major diterpenes isolated from Danshen, and show cytotoxic effects on cell lines derived from human carcinomas of the colon, ovary, lung, mouth, and breast. Recently, anti-cancer activities of tanshinone IIA have been reported, which suggest that the structurally similar tanshinone I may possess similar cytotoxic effects on tumor cells. We investigated the effect of tanshinone I on the induction of apoptosis in human breast cancer cells (MCF-7 and MDA-MB-231) in vitro. Tanshinone I inhibited cell proliferation of MCF-7 and MDA-MB-231 cells in a dose- and time-dependent manner, as assayed by MTT. In addition, TUNEL assay and flow cytometry showed that tanshinone I significantly induced apoptosis in MCF-7 and MDA-MB-231 cells. The induction of apoptotic cell death was mediated by the activation of caspase 3, the downregulation of the level of the anti-apoptotic protein, Bcl-2, and the upregulation of the level of the pro-apoptotic protein, Bax. Taken together, these results reveal a potential mechanism for the anti-cancer effect of tanshinone I on human breast cancer cells, and suggest that tanshinone I may serve as an effective adjunctive reagent in the treatment of human breast cancer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1019-6439
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
485-91
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:18695877-Antineoplastic Agents, Phytogenic, pubmed-meshheading:18695877-Apoptosis, pubmed-meshheading:18695877-Blotting, Western, pubmed-meshheading:18695877-Breast Neoplasms, pubmed-meshheading:18695877-Caspase 3, pubmed-meshheading:18695877-Cell Line, Tumor, pubmed-meshheading:18695877-Cell Proliferation, pubmed-meshheading:18695877-Diterpenes, Abietane, pubmed-meshheading:18695877-Enzyme Activation, pubmed-meshheading:18695877-Female, pubmed-meshheading:18695877-Flow Cytometry, pubmed-meshheading:18695877-Humans, pubmed-meshheading:18695877-In Situ Nick-End Labeling, pubmed-meshheading:18695877-Phenanthrenes, pubmed-meshheading:18695877-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:18695877-Receptors, Estrogen, pubmed-meshheading:18695877-bcl-2-Associated X Protein
pubmed:year
2008
pubmed:articleTitle
Tanshinone I effectively induces apoptosis in estrogen receptor-positive (MCF-7) and estrogen receptor-negative (MDA-MB-231) breast cancer cells.
pubmed:affiliation
Department of Pharmacology, School of Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't