Linked Life Data

18694314

Source:http://linkedlifedata.com/resource/pubmed/id/18694314

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-8-12
pubmed:abstractText
Aims: Herein, we report our work to improve the detection sensitivity of a DNA-tile-based and self-assembled biosensing platform. This was achieved using hybridization chain reaction (HCR) as a signal amplifier on a water-soluble self-assembled DNA nanoarray carrying detection probes. Materials & methods: The fluorescence enhancement on the addition of specific detection targets was observed directly by confocal fluorescence microscopy. Results & discussion: The versatility of the system was demonstrated by successful detection of SARS viral DNA and ATP. Improvement of sensitivity by two orders of magnitude was achieved for both targets, compared with our previously reported system without signal amplification. Conclusion: In summary, this work provides proof-of-concept that HCR can occur efficiently on DNA-tile-based nanoarrays, thus facilitating more sensitive detection. The merging of HCR with combinatorial encoding systems to realize highly sensitive and multiplexed biosensing may provide new tools for nanomedical applications.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1748-6963
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
521-8
pubmed:dateRevised
2010-4-29
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity.
pubmed:affiliation
Department of Chemistry and Biochemistry, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural