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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
2008-10-1
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pubmed:abstractText |
Heat shock protein 90alpha (Hsp90alpha) is a molecular chaperone that has been targeted for the development of new anticancer therapies. To date, co-immunoprecipitation (IP) has been primarily used to identify novel client proteins. We now report an alternative approach in which Hsp90alpha has been immobilized onto the surface of silica-based magnetic beads. The beads were used to isolate known Hsp90alpha ligands from a mixture containing ligands and nonligands. In addition, they were also used to isolated proteins from a mixture of proteins, as well as a cellular extract. The results indicate that the Hsp90alpha coated magnetic beads can be used to "fish" from complex chemical and biological mixtures for new lead drug candidates and client proteins.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-10945979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-11060043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-11988487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-12161444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-14508491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-16263121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-16483782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-17536171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-17579480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-18047824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18693748-5432063
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/HSP90 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immobilized Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Multiprotein Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Novobiocin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/geldanamycin
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1520-6882
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7571-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18693748-Adenosine Triphosphate,
pubmed-meshheading:18693748-Benzoquinones,
pubmed-meshheading:18693748-HSP90 Heat-Shock Proteins,
pubmed-meshheading:18693748-Immobilized Proteins,
pubmed-meshheading:18693748-Lactams, Macrocyclic,
pubmed-meshheading:18693748-Ligands,
pubmed-meshheading:18693748-Magnetics,
pubmed-meshheading:18693748-Multiprotein Complexes,
pubmed-meshheading:18693748-Novobiocin,
pubmed-meshheading:18693748-Protein Binding,
pubmed-meshheading:18693748-Proteomics,
pubmed-meshheading:18693748-Recombinant Proteins
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pubmed:year |
2008
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pubmed:articleTitle |
Ligand and protein fishing with heat shock protein 90 coated magnetic beads.
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pubmed:affiliation |
Gerontology Research Center, National Institute on Aging/NIH, Baltimore, Maryland 21224, USA.
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pubmed:publicationType |
Journal Article
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