|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2008-8-26
|
|
pubmed:abstractText |
Evaluation of the therapeutic potential of RNAi for HIV infection has been hampered by the challenges of siRNA delivery and lack of suitable animal models. Using a delivery method for T cells, we show that siRNA treatment can dramatically suppress HIV infection. A CD7-specific single-chain antibody was conjugated to oligo-9-arginine peptide (scFvCD7-9R) for T cell-specific siRNA delivery in NOD/SCIDIL2rgamma-/- mice reconstituted with human lymphocytes (Hu-PBL) or CD34+ hematopoietic stem cells (Hu-HSC). In HIV-infected Hu-PBL mice, treatment with anti-CCR5 (viral coreceptor) and antiviral siRNAs complexed to scFvCD7-9R controlled viral replication and prevented the disease-associated CD4 T cell loss. This treatment also suppressed endogenous virus and restored CD4 T cell counts in mice reconstituted with HIV+ peripheral blood mononuclear cells. Moreover, scFvCD7-9R could deliver antiviral siRNAs to naive T cells in Hu-HSC mice and effectively suppress viremia in infected mice. Thus, siRNA therapy for HIV infection appears to be feasible in a preclinical animal model.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1097-4172
|
|
pubmed:author |
pubmed-author:BanHong-SeokHS,
pubmed-author:FeyGeorg HGH,
pubmed-author:GreinerDale LDL,
pubmed-author:HabiroKatsuyoshiK,
pubmed-author:HaridasViragaV,
pubmed-author:JeongJi-HoonJH,
pubmed-author:KimSang-SooSS,
pubmed-author:KimSung WanSW,
pubmed-author:KimYong-HeeYH,
pubmed-author:KumarPritiP,
pubmed-author:LaouarAmaleA,
pubmed-author:LeeKuen-YongKY,
pubmed-author:LeeSang-KyungSK,
pubmed-author:ManjunathNN,
pubmed-author:PearsonToddT,
pubmed-author:PeippMatthiasM,
pubmed-author:ShankarPremlataP,
pubmed-author:ShultzLeonard DLD,
pubmed-author:WuHaoquanH,
pubmed-author:YangYong-GuangYG,
pubmed-author:YaoJiahongJ
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
22
|
|
pubmed:volume |
134
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
577-86
|
|
pubmed:dateRevised |
2011-8-1
|
|
pubmed:meshHeading |
pubmed-meshheading:18691745-Animals,
pubmed-meshheading:18691745-Antigens, CD7,
pubmed-meshheading:18691745-Disease Models, Animal,
pubmed-meshheading:18691745-Gene Expression,
pubmed-meshheading:18691745-HIV Infections,
pubmed-meshheading:18691745-HIV-1,
pubmed-meshheading:18691745-Humans,
pubmed-meshheading:18691745-Immunoglobulin Fragments,
pubmed-meshheading:18691745-Immunoglobulin Variable Region,
pubmed-meshheading:18691745-Leukocytes, Mononuclear,
pubmed-meshheading:18691745-Mice,
pubmed-meshheading:18691745-Mice, Inbred NOD,
pubmed-meshheading:18691745-Mice, SCID,
pubmed-meshheading:18691745-RNA, Small Interfering,
pubmed-meshheading:18691745-RNA, Viral,
pubmed-meshheading:18691745-RNA Interference,
pubmed-meshheading:18691745-T-Lymphocytes
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
T cell-specific siRNA delivery suppresses HIV-1 infection in humanized mice.
|
|
pubmed:affiliation |
Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
