rdf:type |
|
lifeskim:mentions |
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0025252,
umls-concept:C0162736,
umls-concept:C0205314,
umls-concept:C0441513,
umls-concept:C0679622,
umls-concept:C0870432,
umls-concept:C1167331,
umls-concept:C1514562,
umls-concept:C1527177,
umls-concept:C1555465,
umls-concept:C1705417
|
pubmed:issue |
12
|
pubmed:dateCreated |
2008-10-23
|
pubmed:abstractText |
A novel bacterial cell-surface display system was developed in Escherichia coli using omp1, a hypothetical outer membrane protein of Zymomonas mobilis. By using this system, we successfully expressed beta-amylase gene of sweet potato in E. coli. The display of enzyme on the membrane surface was also confirmed. The recombinant beta-amylase showed to significantly increase hydrolytic activity toward soluble starch. Our results provide a basis for constructing an engineered Z. mobilis strain directly fermenting raw starch to produce ethanol.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
1573-6776
|
pubmed:author |
|
pubmed:issnType |
Electronic
|
pubmed:volume |
30
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2111-7
|
pubmed:meshHeading |
pubmed-meshheading:18688577-Amino Acid Motifs,
pubmed-meshheading:18688577-Bacterial Outer Membrane Proteins,
pubmed-meshheading:18688577-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:18688577-Escherichia coli,
pubmed-meshheading:18688577-Ethanol,
pubmed-meshheading:18688577-Gene Expression,
pubmed-meshheading:18688577-Ipomoea batatas,
pubmed-meshheading:18688577-Membrane Proteins,
pubmed-meshheading:18688577-Recombinant Proteins,
pubmed-meshheading:18688577-Subcellular Fractions,
pubmed-meshheading:18688577-Zymomonas,
pubmed-meshheading:18688577-beta-Amylase
|
pubmed:year |
2008
|
pubmed:articleTitle |
Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif.
|
pubmed:affiliation |
Sichuan Key Laboratory of Molecular Biology & Biotechnology, Key Laboratory of Resource Biology & Eco-environment of Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|