Source:http://linkedlifedata.com/resource/pubmed/id/18688574
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
|
pubmed:dateCreated |
2008-10-23
|
pubmed:abstractText |
A recombinant laccase from Trametes versicolor in Pichia methanolica was produced constitutively in a defined medium. The recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. The molecular weight of the purified laccase was estimated as 64 kDa by SDS-PAGE. The purified recombinant laccase decolorized more than 90% of Remazol Brilliant Blue R (RBBR) initially at 80 mg l(-1) after 16 h at 45 degrees C and pH 5 when 25 U laccase ml(-1) was used. The purified recombinant laccase could efficiently decolorize RBBR without additional redox mediators.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anthraquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Laccase,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Remazol Brilliant Blue R
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
1573-6776
|
pubmed:author | |
pubmed:issnType |
Electronic
|
pubmed:volume |
30
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2091-6
|
pubmed:meshHeading |
pubmed-meshheading:18688574-Anthraquinones,
pubmed-meshheading:18688574-Basidiomycota,
pubmed-meshheading:18688574-Chromatography, Gel,
pubmed-meshheading:18688574-Chromatography, Ion Exchange,
pubmed-meshheading:18688574-Coloring Agents,
pubmed-meshheading:18688574-Laccase,
pubmed-meshheading:18688574-Pichia,
pubmed-meshheading:18688574-Recombinant Proteins,
pubmed-meshheading:18688574-Ultrafiltration
|
pubmed:year |
2008
|
pubmed:articleTitle |
Purification of recombinant laccase from Trametes versicolor in Pichia methanolica and its use for the decolorization of anthraquinone dye.
|
pubmed:affiliation |
Department of Food Science, Tianjin Agriculture College, Tianjin, 300384, People's Republic of China.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|