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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1991-9-18
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pubmed:abstractText |
Very closely related short sequences are present at the 5' end of cytoplasmic mRNAs in Euglena as evidenced by comparison of cDNA sequences and hybrid-arrested translation experiments. By cloning Euglena gracilis nuclear DNA and isolating the rbcS gene (encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase), we have shown that the short leader sequence does not flank the nuclear gene sequence. The leader sequences were found to constitute the 5' extremities of a family of small RNAs. Sequencing six members of this family revealed a striking similarity to vertebrate U snRNAs. We propose that a trans-splicing mechanism transfers the spliced leader (SL) sequence from these small RNAs (SL RNAs) to pre-mature mRNAs. Transfer of leader sequences to mRNAs by trans-splicing has been shown only in trypanosomes where cis-splicing is unknown, and in nematodes where not more than 10% of the mRNAs have leader sequences. Our results strongly suggest that Euglena is a unique organism in which both a widespread trans-splicing and a cis-splicing mechanism co-exist.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-1692263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2105882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2106666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2142018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2339054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2443794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2458850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2477247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2506525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2675423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2680571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2686121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2813415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2911720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-2971142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-3022935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-3031608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-3070853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-3141775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-3203906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-3309569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-3581169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-3737413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-6091897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-6094183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-6287414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1868836-7433111
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:geneSymbol |
rbcS
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2621-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1868836-Animals,
pubmed-meshheading:1868836-Base Sequence,
pubmed-meshheading:1868836-Blotting, Northern,
pubmed-meshheading:1868836-DNA, Protozoan,
pubmed-meshheading:1868836-Euglena,
pubmed-meshheading:1868836-Molecular Sequence Data,
pubmed-meshheading:1868836-Nucleic Acid Hybridization,
pubmed-meshheading:1868836-RNA, Messenger,
pubmed-meshheading:1868836-RNA, Protozoan,
pubmed-meshheading:1868836-RNA, Small Nuclear,
pubmed-meshheading:1868836-RNA Splicing,
pubmed-meshheading:1868836-Restriction Mapping,
pubmed-meshheading:1868836-Ribulose-Bisphosphate Carboxylase
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pubmed:year |
1991
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pubmed:articleTitle |
Short leader sequences may be transferred from small RNAs to pre-mature mRNAs by trans-splicing in Euglena.
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pubmed:affiliation |
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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