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rdf:type |
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lifeskim:mentions |
umls-concept:C0003316,
umls-concept:C0004611,
umls-concept:C0021311,
umls-concept:C0024141,
umls-concept:C0208973,
umls-concept:C0233577,
umls-concept:C0282676,
umls-concept:C1517892,
umls-concept:C1527178,
umls-concept:C1704666,
umls-concept:C1705938
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pubmed:dateCreated |
2008-8-6
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pubmed:abstractText |
We previously demonstrated that purified polyclonal and monoclonal anti-dsDNA antibodies bind a 15-mer peptide ASPVTARVLWKASHV in ELISA and Dot blot. This 15-mer peptide partial sequence ARVLWKASH shares similarity with burkholderia bacterial cytochrome B 561 partial sequence ARVLWRATH. In this study, we show that purified anti-dsDNA antibodies react with burkholderia fungorum bacterial cell lysates in Western blot. We used anti-dsDNA antibodies to make an anti-dsDNA antibodies affinity column and used this column to purify the burkholderia fungorum bacterial protein. Purified anti-dsDNA antibodies bind specifically to purified bacterial antigen and purified bacterial antigen blocked the anti-dsDNA antibodies binding to dsDNA antigen. Sera with anti-dsDNA antibodies bind specifically to purified bacterial antigen. We obtained protein partial sequence of RAGTDEGFG which is shared with burkholderia bacterial transcription regulator protein sequence. Sera with anti-dsDNA antibodies bind to RAGTDEGFG peptide better than control groups. These data support our hypothesis that the origin of anti-dsDNA antibodies in SLE may be associated with burkholderia bacterial infection.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-10564545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-10588732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-11161987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-11411678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-1185733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-1501424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-1626077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-16290070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-16455583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-17153849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-3102132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-3301096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-3308510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-3486968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-3923882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-6420530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-7138600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-7392958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-7576084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-7682645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-855960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18682819-9759550
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1740-2530
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
2008
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
683489
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
A possible link between infection with burkholderia bacteria and systemic lupus erythematosus based on epitope mimicry.
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pubmed:affiliation |
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
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pubmed:publicationType |
Journal Article
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