Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
42
pubmed:dateCreated
2008-10-13
pubmed:abstractText
Activation of the transcriptional regulator SlyA by the PhoP/PhoQ two-component system controls intracellular expression of numerous factors influencing Salmonella virulence. By dissecting the SlyA regulon using stable isotope labeling with amino acids in cell culture analysis, we found that SlyA enhances overall transcription of PhoP-activated loci. This amplification of cellular responses to Mg2+ occurs when SlyA binds to the phoPQ promoter thereby activating phoP autoregulation via a positive feedback mechanism. SlyA footprints a DNA region located one helical turn upstream of the PhoP box, which overlaps the H-NS-binding motif required for signal-dependent phoP repression in high Mg2+ conditions. Therefore, binding of SlyA likely antagonizes H-NS and facilitates the interaction of PhoP to its own promoter, subsequently activating the phoPQ operon. Establishment of this regulatory circuit allows SlyA to exert its effect on the PhoP/PhoQ system specifically in Salmonella, which may confer an additional transcriptional regulation. Thus, our results provide a molecular mechanism that determines SlyA-dependent activation of PhoP-regulated genes in modulating Salmonella virulence. Evidence from this study also suggests a function of SlyA as a mediator in signal transduction from the PhoP/PhoQ system to other bacterial two-component systems in Salmonella.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-10464230, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-10775270, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-10829079, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-11755417, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-11882648, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-12062810, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-12118079, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-12675799, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-12676988, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-12775687, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-15126461, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-15208313, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-15225317, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-15569664, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-15589755, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-15813739, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-16096064, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-1660927, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-16938894, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-17466620, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-1824701, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-18434315, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-2542933, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-374361, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-6263866, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-6345791, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-7543474, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-7789817, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-781293, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-7883164, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-8086001, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-8290552, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-8548821, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-8752324, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-8892844, http://linkedlifedata.com/resource/pubmed/commentcorrection/18678876-9284144
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
283
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28158-68
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Modulation of the regulatory activity of bacterial two-component systems by SlyA.
pubmed:affiliation
Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't