Linked Life Data

186774

Source:http://linkedlifedata.com/resource/pubmed/id/186774

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1977-1-29
pubmed:abstractText
Infection of primary cultures of mouse kidney cells with polyoma virus causes a biphasic increase in the activities of L-ornithine decarboxylase (ODC; L-ornithine carboxy-lyase; EC 4.1.1.17) and S-adenosyl-L-methionine decarboxylase (SAMD; S-adenoxyl-L-methionine carboxy-lyase; EC 4.1.50), as well as in the level of the polyamines putrescine, spermidine, and spermine. An early peak occurs during the period when early viral mRNA is synthesized and prior to the onset of virus-induced synthesis of host cell DNA. A late peak coincides in time with the maximum rate of virus-induced synthesis of cellular DNA. A similar biphasic stimulation of polyamine synthesis is induced even when DNA synthesis is prevented by 5-fluorodeoxyuridine. Actinomycin D (AMD) in a dose that inhibits rRNA synthesis causes no inhibition of ODC or SAMD. In a dose that inhibits mRNA synthesis as well, short-term AMD treatment causes "superinduction" of ODC but inhibition of SAMD. Prolonged treatment with the high dose of AMD inhibits ODC as well, indicating that late ODC activity may be dependent on mRNA synthesized during early infection. Cycloheximide effectively obliterates the ODC and SAMD activities during the entire infectious cycle. Uncoupling from DNA and rRNA synthesis suggests that polyamine synthesis is regulated independently of these events. The experiments with AMD and cycloheximide suggest that the formation of ODC is subject to post-transcriptional control, whereas that of SAMD is regulated primarily at the transcriptional level.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-1060087, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-1168141, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-1202031, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-14001211, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-167737, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-169084, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-17655040, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-4106524, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-4291934, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-4313194, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-4349721, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-4367850, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-4386986, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-4404031, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-4473203, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-806079, http://linkedlifedata.com/resource/pubmed/commentcorrection/186774-962932
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4022-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Biphasic stimulation of polyamine biosynthesis in primary mouse kidney cells by infection with polyoma virus:uncoupling from DNA and rRNA synthesis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.