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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2008-10-2
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pubmed:abstractText |
We previously reported that the barrier-protective effects of oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (OxPAPC) on pulmonary endothelial cells (ECs) delineate the role of Rac- and Cdc42-dependent mechanisms and described the involvement of the focal adhesion (FA) protein paxillin in enhancement of the EC barrier upon OxPAPC challenge. This study examined a potential role of paxillin in the feedback mechanism of Rac regulation by FAs in OxPAPC-stimulated ECs. Our results demonstrate that OxPAPC induced Rac-dependent, Rho-independent peripheral accumulation of paxillin-containing FAs and time-dependent paxillin phosphorylation. Molecular inhibition of Rac decreased association of paxillin with the Rac-specific guanine nucleotide exchange factor beta-PIX. Molecular inhibition of paxillin also attenuated OxPAPC-induced enhancement of adherens junctions critical for the EC barrier-protective response, accumulation of vascular endothelial cadherin in the membrane fractions, and decreased activation of Rac and its effector p21-activated kinase (PAK1). Expression of paxillin with a mutated PAK1-dependent phosphorylation site (S273A) attenuated OxPAPC-induced PAK1 activation and the EC barrier-protective response. These results suggest that PAK1-specific paxillin phosphorylation at Ser(273) is critically involved in the positive-feedback regulation of the Rac-PAK1 pathway and may contribute to sustained enhancement of the EC barrier caused by oxidized phospholipids.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-10518567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-10938112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11069756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11146675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11402062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11448998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11544028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11607845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11756172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11897849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-11944043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12006652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12214235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12446743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12471085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12475808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12482769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12570126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12639843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12676796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-12943964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-13679995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-14501580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-14644758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-14656986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-14709399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-14709404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-14743221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15281089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15292228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15292510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15308497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15383653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15472119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15576491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15688067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15707572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15935342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-15972685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-16000375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-16257999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-16514111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-16543511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-16640959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-16651639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-16717130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-17012369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-17158600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-17219408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-17292425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-17493609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-17513457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-7615549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-9153208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18676874-9596132
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1040-0605
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
295
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L593-602
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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