Linked Life Data

18675338

Source:http://linkedlifedata.com/resource/pubmed/id/18675338

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-9-22
pubmed:abstractText
The physiological role of microcystin-LR is still under discussion, and since binding of microcystin-LR to proteins different from their main cellular targets was described, we have performed experiments in order to explore this interaction. A non-specific interaction of microcystin-LR with a variety of soluble proteins in vitro is disrupted when using organic solvents such as methanol. The isoelectric point of proteins is not affected by their interaction with microcystin-LR, even though the presence of microcystin-LR alters the pool of peptides obtained by tryptic digestions. Under the conditions tested, microcystin-LR does not exhibit affinity for DNA. Although it is unlikely that the non-specific binding of microcystin-LR to proteins has a physiological meaning, one must be aware of the fact that determinations of the toxin extracted from any biological sample may be affected by the presence of proteins in the extracts. Consequently, we strongly recommend use organic solvents and to lyophilise the tissue samples to guarantee the accessibility of these organic solvents to microcystin-LR when performing experiments with tissue or cell extracts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0887-2333
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1714-8
pubmed:dateRevised
2009-4-10
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Exploring the interaction of microcystin-LR with proteins and DNA.
pubmed:affiliation
Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Ciencias, and BIFI, Universidad de Zaragoza, Pedro Cerbuna 12, 50009-Zaragoza, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't