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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-12-3
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pubmed:abstractText |
Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and msa-mutant strain had been applied. In contrast to the mutant strains, the L. plantarum wild-type strain tended to induce a decrease in jejunal net fluid absorption compared with control conditions. Furthermore, after 8 h of perfusion expression of the host gene encoding pancreatitis-associated protein, a protein with proposed bactericidal properties, was found to be upregulated by the wild-type strain only. These observations suggest a role of Msa in the induction of host responses in the pig intestine.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adhesins, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Aminoacyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/adhesin, Lactobacillus,
http://linkedlifedata.com/resource/pubmed/chemical/pancreatitis-associated protein,
http://linkedlifedata.com/resource/pubmed/chemical/sortase A
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0928-8244
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-23
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pubmed:dateRevised |
2010-2-8
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pubmed:meshHeading |
pubmed-meshheading:18673389-Adhesins, Bacterial,
pubmed-meshheading:18673389-Aminoacyltransferases,
pubmed-meshheading:18673389-Animals,
pubmed-meshheading:18673389-Antigens, Neoplasm,
pubmed-meshheading:18673389-Bacterial Proteins,
pubmed-meshheading:18673389-Cysteine Endopeptidases,
pubmed-meshheading:18673389-Intestinal Absorption,
pubmed-meshheading:18673389-Intestinal Mucosa,
pubmed-meshheading:18673389-Jejunum,
pubmed-meshheading:18673389-Lactobacillus plantarum,
pubmed-meshheading:18673389-Lectins, C-Type,
pubmed-meshheading:18673389-Male,
pubmed-meshheading:18673389-Mannose,
pubmed-meshheading:18673389-Models, Animal,
pubmed-meshheading:18673389-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:18673389-Probiotics,
pubmed-meshheading:18673389-Swine,
pubmed-meshheading:18673389-Tumor Markers, Biological
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pubmed:year |
2008
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pubmed:articleTitle |
Mannose-specific interaction of Lactobacillus plantarum with porcine jejunal epithelium.
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pubmed:affiliation |
Animal Breeding and Genomics Centre, Animal Sciences Group of Wageningen UR, Lelystad, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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