Source:http://linkedlifedata.com/resource/pubmed/id/18673329
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2009-1-20
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| pubmed:abstractText |
Twenty-four ejaculates from six (four ejaculates each) Surti buffalo bulls aged 4-8 years were used to assess various attributes of spermatozoa influencing the zona-binding and zona-penetration tests. Ejaculates from each bulls were subjected to in vitro sperm--zona binding and sperm--zona penetration tests (four replicates per bull) using immature buffalo oocytes. The average number of spermatozoa bound per oocyte was 27.79 +/- 5.90. The average number of spermatozoa penetrated per oocyte was 3.35 +/- 0.64. The average number of zona-bound and -penetrated spermatozoa differed significantly between animals. Significant difference (p < 0.05) was observed between the plasmalemma integrity as assessed by eosin--nigrosin stain and hypo-osmotic swelling (HOS) test. Furthermore, the percentage of cells positive for the HOS test, i.e. functional membrane integrity (51.25 +/- 2.32) was significantly (p < 0.05) higher than hypo-osmotic swelling-Giemsa (HOS-G) test, i.e. the subpopulation of spermatozoa positive for functional membrane and acrosomal integrities (42.87 +/- 4.56). The HOS test had significant correlations with plasmalemma integrity as measured by the vital stain, eosin--nigrosin (r = 0.85, p < 0.05). The HOS-G test also had significant correlation with plasmalemma integrity measured by vital stains such as eosin--nigrosin (r = 0.90, p < 0.05) and fluorogenic stains [carboxyfluorescein diacetate (CFDA) and propidium iodide (PI); r = 0.92, p < 0.01] and HOS test (r = 0.93), acrosomal integrity (r = 0.86, p < 0.05) and mitochondrial membrane potential (r = 0.99, p < 0.01). The plasmalemma integrity (fluorogenic stain), functional membrane integrity (HOS test), subpopulation of spermatozoa positive for functional membrane and acrosomal integrities (HOS-G test) and mitochondrial membrane potential had significant (p < 0.05) correlation with sperm zona binding and penetration. The present study indicates that these parameters could represent important determinants of sperm quality influencing zona binding and penetration.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1439-0531
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
44
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
6-11
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| pubmed:meshHeading |
pubmed-meshheading:18673329-Acrosome,
pubmed-meshheading:18673329-Animals,
pubmed-meshheading:18673329-Buffaloes,
pubmed-meshheading:18673329-Cell Membrane,
pubmed-meshheading:18673329-Female,
pubmed-meshheading:18673329-Fertility,
pubmed-meshheading:18673329-Insemination, Artificial,
pubmed-meshheading:18673329-Male,
pubmed-meshheading:18673329-Membrane Potential, Mitochondrial,
pubmed-meshheading:18673329-Microscopy, Fluorescence,
pubmed-meshheading:18673329-Oocytes,
pubmed-meshheading:18673329-Osmotic Pressure,
pubmed-meshheading:18673329-Prognosis,
pubmed-meshheading:18673329-Semen,
pubmed-meshheading:18673329-Sperm Motility,
pubmed-meshheading:18673329-Sperm-Ovum Interactions,
pubmed-meshheading:18673329-Spermatozoa
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| pubmed:year |
2009
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| pubmed:articleTitle |
Prognostic value of various spermatological attributes as predictors of zona binding and zona penetration of buffalo (Bubalus bubalis) semen.
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| pubmed:affiliation |
Reproductive Physiology Laboratory, Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Bangalore, India. selvarajuars@rediffmail.com
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| pubmed:publicationType |
Journal Article
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