rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1977-1-25
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pubmed:abstractText |
The association of vesicular stomatitis virus proteins with intracellular and plasma membranes was examined by pulse and pulse-chase labeling of virus-infected HeLa cells with [35S]methionine and separation of cell homogenates into three major membrane fractions in discontinuous sucrose gradients. The glycoprotein G was primarily associated with rough endoplasmic reticulum-like membranes after short radioactive pulses (2 to 4 min) but accumulated in the plasma membrane-enriched fraction and the smooth internal membrane fraction with longer pulse or chase periods. The nucleocapsid protein N and the matrix protein M accumulated in the rough endoplasmic reticulum and plasma membrane-like fractions but not in the smooth internal membrane fraction. Only a fraction (35 to 40%) of the viral protein synthesized during a short pulse in the mid-cycle of infection was apparently utilized in released virus. The newly synthesized virus proteins first appeared in released virus in the order: M, N and L, and G.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-1117000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-14449377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-163229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-163253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-164182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-169740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-178880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-186640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4316780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4321299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4322870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4323715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4325710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4332974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4336563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4345495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4352411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4355329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4355931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4361461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4366497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4368799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4372395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4413592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-4844421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-5698029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/186639-5748826
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Dec
|
pubmed:issn |
0022-538X
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
20
|
pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
637-45
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
|
pubmed:year |
1976
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pubmed:articleTitle |
Association of vesicular stomatitis virus proteins with HeLa cell membranes and released virus.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|