Linked Life Data

18662726

Source:http://linkedlifedata.com/resource/pubmed/id/18662726

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-9-8
pubmed:abstractText
We developed a novel PCR-based method for coagulase serotyping. Coagulase gene amplicons biotinylated by PCR were identified by microplate hybridization (MPH) using serotype-specific probes. The conventional serotyping method, which is strongly dependent on coagulase activity, may sometimes give a mistaken determination of the serotype, especially in cases where there is high coagulase activity. In contrast, the results of PCR-MPH are not affected by coagulase activity. Furthermore, once the isolated colonies are obtained, it only takes 3 h to perform PCR-MPH, and the interpretation of the results is entirely objective. We compared PCR-MPH with the conventional method for 90 strains of coagulase-producing Staphylococcus aureus. The same results were found for both the PCR-MPH and conventional methods, and thus, our results indicate that PCR-MPH is a rapid, objective, and reliable method for coagulase serotyping.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0167-7012
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
308-11
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Development of rapid coagulase serotyping method by PCR and microplate hybridization.
pubmed:affiliation
Fukuyama City Public Health Center, 2-11-22, Miyoshi-chou-minami, Fukuyama, Hiroshima 720-0032, Japan. shiken-kensa@city.fukuyama.hiroshima.jp
pubmed:publicationType
Journal Article, Evaluation Studies