Source:http://linkedlifedata.com/resource/pubmed/id/18662588
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2008-7-29
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pubmed:abstractText |
The cyclin-dependent kinase (cdk) inhibitor p27(Kip1) is an important regulator of cell cycle progression as it negatively regulates G(0/1) progression and plays a major role in controlling the cell cycle. The screening of the p27(Kip1) sequence identified many potential phosphorylation sites. To investigate the effects of the overexpression of exogenous p27(Kip1) protein lacking the Thr157 sites on subcellular localization, cell cycle, and proliferation, a plasmid was constructed containing mutations of p27(Kip1) at Thr157 (T157A p27), and transfected into the SMMC7721 cell line with Lipofectamine. Wild-type and mutant p27 plasmids T157A were transfected separately as control groups.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0188-4409
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
573-81
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pubmed:meshHeading |
pubmed-meshheading:18662588-Base Sequence,
pubmed-meshheading:18662588-Carcinoma, Hepatocellular,
pubmed-meshheading:18662588-Cell Line, Tumor,
pubmed-meshheading:18662588-Cyclin-Dependent Kinase Inhibitor p27,
pubmed-meshheading:18662588-DNA Primers,
pubmed-meshheading:18662588-Fluorescent Antibody Technique,
pubmed-meshheading:18662588-Gene Therapy,
pubmed-meshheading:18662588-Humans,
pubmed-meshheading:18662588-Liver Neoplasms,
pubmed-meshheading:18662588-Mutagenesis, Site-Directed,
pubmed-meshheading:18662588-Mutation,
pubmed-meshheading:18662588-Subcellular Fractions
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pubmed:year |
2008
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pubmed:articleTitle |
Mutant p27(Kip1) and its potential effect as hepatocellular gene therapy.
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pubmed:affiliation |
Department of Pathology, Affiliated Cancer Hospital of Nantong University, Medical College of Nantong University, Nantong, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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