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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2008-7-28
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pubmed:abstractText |
An effective approach was proposed to the derivatization of seven biogenic amines using 3-(4-fluorobenzoyl)-2-quinolinecarboxaldehyde (FBQCA) as a fluorogenic reagent. The sensitive determinations of these derivatives were achieved by micellar electrokinetic capillary chromatography (MEKC) with laser-induced fluorescence (LIF) detection. The derivatization and electrophoretic conditions have been optimized. A running buffer was composed of mixtures of 25 mM pH 9.5 boric acid, 25 mM SDS, and 27% ACN. At 25 degrees C and 22.5 kV, the baseline separation of the derivatives was accomplished in 13 min. The detection limit (S/N=3) was found as low as 0.4 nM. The proposed method was validated by the linearity of two orders magnitude and correlation coefficient in the range 0.9969-0.9998. Also, the procedure was successfully applied to the determination of biogenic amines in soy sauce, fish and wine samples.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1873-3573
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
791-7
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pubmed:meshHeading |
pubmed-meshheading:18656660-Benzoates,
pubmed-meshheading:18656660-Biogenic Amines,
pubmed-meshheading:18656660-Chromatography,
pubmed-meshheading:18656660-Electrochemistry,
pubmed-meshheading:18656660-Electrophoresis, Capillary,
pubmed-meshheading:18656660-Equipment Design,
pubmed-meshheading:18656660-Fluorescent Dyes,
pubmed-meshheading:18656660-Food Contamination,
pubmed-meshheading:18656660-Humans,
pubmed-meshheading:18656660-Hydrogen-Ion Concentration,
pubmed-meshheading:18656660-Kinetics,
pubmed-meshheading:18656660-Lasers,
pubmed-meshheading:18656660-Micelles,
pubmed-meshheading:18656660-Quinolines,
pubmed-meshheading:18656660-Reproducibility of Results,
pubmed-meshheading:18656660-Temperature
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pubmed:year |
2008
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pubmed:articleTitle |
Sensitive determination of biogenic amines by capillary electrophoresis with a new fluorogenic reagent 3-(4-fluorobenzoyl)-2-quinolinecarboxaldehyde.
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pubmed:affiliation |
Department of Chemistry, Wuhan University, Wuhan 430075, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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