Linked Life Data

18656366

Source:http://linkedlifedata.com/resource/pubmed/id/18656366

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2008-8-18
pubmed:abstractText
Polymethoxy flavones (PMFs) are present in fruit tissues of Citrus species. It has been reported that flavonoids isolated from several Citrus have been shown to suppress the degranulation as inferred by histamine release in rat basophilic leukemia RBL-2H3 cells. In this study, we examined the effect of PMFs (PMF-1: 6,7,4',5'-tetramethoxy-5-monohydroxyflavone, PMF-2: 5,6,8,3',6'-pentamethoxy flavone, PMF-3: 5,6,7,3',4',5'-hexamethoxy flavone) on the degranulation in RBL-2H3 cells. All the PMFs suppressed the degranulation from Ag-stimulated RBL-2H3 cells. Interestingly, PMF-combination (PMF-1+PMF-2; PMF-1+PMF-3) treatment enhanced the inhibition of degranulation compared with PMF-single treatment. In order to clarify the inhibitory mechanism of degranulation by PMFs, we examined the activation of intracellular signaling molecules such as Lyn, Syk, and PLCgammas. All the PMFs significantly suppressed the activation of Syk and PLCgammas. In Ag-mediated activation of Fc epsilonRI on mast cells, three major subfamilies of mitogen-activated protein kinases, especially ERK44/42, were activated. These PMFs reduced the level of phospho-ERKs. The intracellular free Ca(2+) concentration ([Ca(2+)]i) was elevated by Fc epsilonRI activation, and PMF treatment reduced the elevation of [Ca(2+)]i by suppressing Ca(2+) influx. Thus, it was suggested that the suppression of Ag-stimulated degranulation by these PMFs mainly is due to the Syk/PLCgammas/PKC pathway and Ca(2+) influx. Furthermore, to be noted in the PMF-combination treatment, inactivation of Syk was enhanced compared with PMF-single treatment. But the inhibitory effect of degranulation by PMF-combination treatment was not associated with the suppression of Ca(2+) influx.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1464-3391
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7592-8
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed-meshheading:18656366-Animals, pubmed-meshheading:18656366-Basophils, pubmed-meshheading:18656366-Calcium, pubmed-meshheading:18656366-Cell Degranulation, pubmed-meshheading:18656366-Cell Line, Tumor, pubmed-meshheading:18656366-Citrus, pubmed-meshheading:18656366-Enzyme Activation, pubmed-meshheading:18656366-Flavones, pubmed-meshheading:18656366-Histamine Release, pubmed-meshheading:18656366-Immunoblotting, pubmed-meshheading:18656366-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:18656366-Leukemia, Basophilic, Acute, pubmed-meshheading:18656366-Phospholipase C gamma, pubmed-meshheading:18656366-Protein-Tyrosine Kinases, pubmed-meshheading:18656366-Rats, pubmed-meshheading:18656366-Reactive Oxygen Species, pubmed-meshheading:18656366-Receptors, IgG, pubmed-meshheading:18656366-Signal Transduction, pubmed-meshheading:18656366-src-Family Kinases
pubmed:year
2008
pubmed:articleTitle
Inhibitory effects of polymethoxy flavones isolated from Citrus reticulate on degranulation in rat basophilic leukemia RBL-2H3: enhanced inhibition by their combination.
pubmed:affiliation
Gifu International Institute of Biotechnology, 1-1 Naka-Fudogaoka, Kakamigahara, Gifu 504-0838, Japan. titoh@giib.or.jp
pubmed:publicationType
Journal Article