Source:http://linkedlifedata.com/resource/pubmed/id/18656220
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2008-8-18
|
| pubmed:databankReference | |
| pubmed:abstractText |
Guinea pig cytomegalovirus (GPCMV) provides a useful model for studies of congenital CMV infection. During characterization of the GPCMV genome sequence, we identified two types of strains in a virus stock purchased from ATCC. One of them, GPCMV/del, lacks a 1.6 kb locus that positionally corresponds to murine CMV (MCMV) M129-M133. Growth of GPCMV/del in cell culture was marginally better than that of the other strain, GPCMV/full, which harbors the 1.6 kb locus. However, in animals infected intraperitoneally with virus stocks containing both strains, GPCMV/full disseminated more efficiently than GPCMV/del, including 200-fold greater viral load in salivary glands. Viral DNA, transcripts of the immediate-early 2 gene homolog, and viral antigens were more abundant in animals infected with GPCMV/full than in those infected with GPCMV/del. Although the observed phenomena have some similarity with the growth properties of MCMV strains defective in mck-1/mck-2(M129/131) and those defective in sgg(M132), no M129-M132 homologs were found in the 1.6 kb locus. Since one of the ORFs in the locus has a weak sequence similarity with HCMV UL130, which relates to cell tropism, further studies will be required to learn the mechanism for efficient GPCMV growth in animal.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1096-0341
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| pubmed:author |
pubmed-author:FukuiYoshikoY,
pubmed-author:InamiYuhkiY,
pubmed-author:InoueNaokiN,
pubmed-author:KatanoHarutakaH,
pubmed-author:KuraneIchiroI,
pubmed-author:NakamuraKohnosukeK,
pubmed-author:NozawaNaokiN,
pubmed-author:SatoYukoY,
pubmed-author:TsutsuiYoshihiroY,
pubmed-author:YamadaSouichiS,
pubmed-author:YamamotoYumikoY,
pubmed-author:YokoiMasayukiM
|
| pubmed:issnType |
Electronic
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| pubmed:day |
15
|
| pubmed:volume |
379
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
45-54
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:18656220-Animals,
pubmed-meshheading:18656220-Antigens, Viral,
pubmed-meshheading:18656220-Cell Culture Techniques,
pubmed-meshheading:18656220-Cell Line,
pubmed-meshheading:18656220-DNA, Viral,
pubmed-meshheading:18656220-Genome, Viral,
pubmed-meshheading:18656220-Guinea Pigs,
pubmed-meshheading:18656220-Liver,
pubmed-meshheading:18656220-Molecular Sequence Data,
pubmed-meshheading:18656220-Phylogeny,
pubmed-meshheading:18656220-RNA, Viral,
pubmed-meshheading:18656220-Roseolovirus,
pubmed-meshheading:18656220-Roseolovirus Infections,
pubmed-meshheading:18656220-Salivary Glands,
pubmed-meshheading:18656220-Sequence Analysis, DNA,
pubmed-meshheading:18656220-Sequence Deletion,
pubmed-meshheading:18656220-Sequence Homology,
pubmed-meshheading:18656220-Spleen,
pubmed-meshheading:18656220-Viral Plaque Assay,
pubmed-meshheading:18656220-Virus Replication
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| pubmed:year |
2008
|
| pubmed:articleTitle |
Identification of a 1.6 kb genome locus of guinea pig cytomegalovirus required for efficient viral growth in animals but not in cell culture.
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| pubmed:affiliation |
Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|