Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2008-10-28
pubmed:abstractText
Application of basic fibroblast growth factor (FGF-2) to the optic nerve after axotomy promotes the survival of retinal ganglion cells (RGCs) in the frog Rana pipiens and results in a rapid up-regulation of brain-derived neurotrophic factor (BDNF) and TrkB synthesis by the RGCs. Here we investigate whether this up-regulation is maintained over the long term and whether it is required for FGF-2's survival effect. At 6 weeks after axotomy and FGF-2 treatment, we found more RGCs immunopositive for BDNF protein and higher intensity of BDNF and TrkB immunostaining, accompanied by increases in BDNF and TrkB mRNA in RGCs. Application of fluorescently labeled siRNA targeted against BDNF to the cut RGC axons showed that it was transported to the cell bodies. Axonal siRNA treatment eliminated the increases in BDNF immunostaining and mRNA that were induced by FGF-2 and had no effect on TrkB mRNA. This reduction in BDNF synthesis by siRNA greatly reduced the long-term survival effect of FGF-2 on RGCs. This, taken together with previous results, suggests that, although FGF-2 may initially activate survival pathways via ERK signaling, its main long-term survival effects are mediated via its up-regulation of BDNF synthesis by the RGCs.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-10399940, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-10559401, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-10711692, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-10716894, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-10880994, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-11006239, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-11809469, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-12019317, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-12049586, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-12196587, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-12368805, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-12385629, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-12455009, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-14507880, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-15202684, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-15380477, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-15659426, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-15935058, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-16269011, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-16682561, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-17156937, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-17406670, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-3488614, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-3587749, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-3876140, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-7570017, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-7583114, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-7700353, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-8050370, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-8205613, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-8341689, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-8726105, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-8833445, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-8873757, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-9286274, http://linkedlifedata.com/resource/pubmed/commentcorrection/18655198-9589902
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1097-4547
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3382-92
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Up-regulation of brain-derived neurotrophic factor by application of fibroblast growth factor-2 to the cut optic nerve is important for long-term survival of retinal ganglion cells.
pubmed:affiliation
Institute of Neurobiology, University of Puerto Rico Medical Sciences Campus, Old San Juan, Puerto Rico. rblanco@rcm.upr.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural