Source:http://linkedlifedata.com/resource/pubmed/id/18654608
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2008-12-16
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| pubmed:abstractText |
The overexpression of reduced expression in immortalized cells (REIC)/Dickkopf-3 (Dkk-3), a tumor suppressor gene, induced apoptosis in human prostatic and testicular cancer cells. The aim of this study is to examine the potential of REIC/Dkk-3 as a therapeutic target against breast cancer. First, the in vitro apoptotic effect of Ad-REIC treatment was investigated in breast cancer cell lines and the adenovirus-mediated overexpression of REIC/Dkk-3 was thus found to lead to apoptotic cell death in a c-Jun-NH(2)-kinase (JNK) phosphorylaion-dependent manner. Moreover, an in vivo apoptotic effect and MCF/Wt tumor growth inhibition were observed in the mouse model after intratumoral Ad-REIC injection. As multidrug resistance (MDR) is a major problem in the chemotherapy of progressive breast cancer, the in vitro effects of Ad-REIC treatment were investigated in terms of the sensitivity of multidrug-resistant MCF7/ADR cells to doxorubicin and of the P-glycoprotein expression. Ad-REIC treatment in MCF7/ADR cells also downregulated P-glycoprotein expresssion through JNK activation, and sensitized its drug resistance against doxorubicin. Therefore, not only apoptosis induction but also the reversal of anticancer drug resistance was achieved using Ad-REIC. We suggest that REIC/Dkk-3 is a novel target for breast cancer treatment and that Ad-REIC might be an attractive agent against drug-resistant cancer in combination with conventional antineoplastic agents.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/DKK3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/MAP Kinase Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/P-Glycoprotein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1476-5500
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
16
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
65-72
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:18654608-Adenoviridae,
pubmed-meshheading:18654608-Animals,
pubmed-meshheading:18654608-Antibiotics, Antineoplastic,
pubmed-meshheading:18654608-Apoptosis,
pubmed-meshheading:18654608-Breast Neoplasms,
pubmed-meshheading:18654608-Down-Regulation,
pubmed-meshheading:18654608-Doxorubicin,
pubmed-meshheading:18654608-Drug Resistance, Multiple,
pubmed-meshheading:18654608-Drug Resistance, Neoplasm,
pubmed-meshheading:18654608-Enzyme Activation,
pubmed-meshheading:18654608-Female,
pubmed-meshheading:18654608-Gene Therapy,
pubmed-meshheading:18654608-HeLa Cells,
pubmed-meshheading:18654608-Humans,
pubmed-meshheading:18654608-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:18654608-MAP Kinase Kinase 4,
pubmed-meshheading:18654608-Mice,
pubmed-meshheading:18654608-Mice, Nude,
pubmed-meshheading:18654608-Neoplasm Transplantation,
pubmed-meshheading:18654608-P-Glycoprotein
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| pubmed:year |
2009
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| pubmed:articleTitle |
REIC/Dkk-3 overexpression downregulates P-glycoprotein in multidrug-resistant MCF7/ADR cells and induces apoptosis in breast cancer.
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| pubmed:affiliation |
Department of Cancer and Thoracic Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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