Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2008-9-29
pubmed:abstractText
Small GTPases control key cellular events, including formation of cell-cell junctions and gene expression, and are regulated by activating and inhibiting factors. Here, we characterize the junctional protein paracingulin as a novel regulator of the activity of two small GTPases, Rac1 and RhoA, through the functional interaction with their respective activators, Tiam1 and GEF-H1. In confluent epithelial monolayers, paracingulin depletion leads to increased RhoA activity and increased expression of mRNA for the tight junction protein claudin-2. During tight junction assembly by the calcium-switch, Rac1 shows two transient peaks of activity, at earlier (10-20 min) and later (3-8 h) time points. Paracingulin depletion reduces such peaks of Rac1 activation in a Tiam1-dependent manner, resulting in a delay in junction formation. Paracingulin physically interacts with GEF-H1 and Tiam1 in vivo and in vitro, and it is required for their efficient recruitment to junctions, based on immunofluorescence and biochemical experiments. Our results provide the first description of a junctional protein that interacts with GEFs for both Rac1 and RhoA, and identify a novel molecular mechanism whereby Rac1 is activated during junction formation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-10383446, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-10613913, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-10931868, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-10980705, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-11329369, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-11457821, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-11544030, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-11682052, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-11733768, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-11912491, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-12023291, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-12147707, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-12186943, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-12194856, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-12231348, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-12600316, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-12829230, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-15044152, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-15122349, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-15138270, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-15151915, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-15292197, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-15688002, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-15723052, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-15866167, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16025097, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16186252, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16212495, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16339077, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16460278, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16599904, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16678097, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16723500, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-16771626, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-17129786, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-17545772, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-17646397, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-18097951, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-18339298, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-18394899, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-3049625, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-3285223, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-4032460, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-7479854, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-7485497, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-7999144, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-8056850, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-9128250, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-9182672, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-9353304, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-9660866, http://linkedlifedata.com/resource/pubmed/commentcorrection/18653465-9832565
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ARHGEF2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/CLDN2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/TIAM1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/TJP3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/rac1 GTP-Binding Protein, http://linkedlifedata.com/resource/pubmed/chemical/rhoA GTP-Binding Protein
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1939-4586
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4442-53
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
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