|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2008-7-23
|
|
pubmed:abstractText |
Pannexins are mammalian orthologs of innexins and have a predicted topological folding pattern similar to that of connexins, except they are glycosylated. Rat pannexin 1 is glycosylated at N254 and this residue is important for plasma membrane targeting. Here we demonstrate that cell surface expression levels of the rat pannexin 1 N254Q mutant are rescued by coexpression with the wild-type protein. In paired Xenopus oocytes, the functional effect of this rescue is inconsequential; however, cell surface deglycosylation by PNGase F significantly enhanced functional gap junction formation. In mammalian cells, wild-type oligomers traffic at a slower rate than Myc-or tetracysteine domain-tagged versions, a behavior opposite to that of tagged connexins. The temporal differences of Panx1 trafficking correlate with spatial differences of intracellular localizations induced by Golgi blockage by Brefeldin-A or glycosylation prevention by tunicamycin. Therefore, Panx1 has kinetics and dynamics that make it unique to serve distinct functions separate from connexin-based channels.
|
|
pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/GM065937,
http://linkedlifedata.com/resource/pubmed/grant/GM072881,
http://linkedlifedata.com/resource/pubmed/grant/GM48610,
http://linkedlifedata.com/resource/pubmed/grant/P41 RR004050-178230,
http://linkedlifedata.com/resource/pubmed/grant/P41 RR004050-20,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM048610-13A2,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM065937-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM065937-04S1,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM072881-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM072881-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM072881-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 GM072881-04,
http://linkedlifedata.com/resource/pubmed/grant/RR04050
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-10359613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-10706639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-10940315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-11427541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-11964472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-13130072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-14597722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-14688283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-15033586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-15304325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-15802665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-16155565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-16195341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-16492141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-1662902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-16682648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-16801216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-1682055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-16908669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-16989724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-17009242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-17036048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-17121814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-17240370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-17715132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-17925379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-18035059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-2647301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-6259274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-7592880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-7691412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-8522583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18649184-9925876
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
1543-5180
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
15
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
119-32
|
|
pubmed:dateRevised |
2010-12-17
|
|
pubmed:meshHeading |
pubmed-meshheading:18649184-Amino Acid Substitution,
pubmed-meshheading:18649184-Animals,
pubmed-meshheading:18649184-Cell Line,
pubmed-meshheading:18649184-Connexins,
pubmed-meshheading:18649184-Dogs,
pubmed-meshheading:18649184-Follow-Up Studies,
pubmed-meshheading:18649184-Gap Junctions,
pubmed-meshheading:18649184-Glycosylation,
pubmed-meshheading:18649184-Humans,
pubmed-meshheading:18649184-Kinetics,
pubmed-meshheading:18649184-Nerve Tissue Proteins,
pubmed-meshheading:18649184-Protein Structure, Tertiary,
pubmed-meshheading:18649184-Protein Transport,
pubmed-meshheading:18649184-Rats
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Trafficking dynamics of glycosylated pannexin 1 proteins.
|
|
pubmed:affiliation |
National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, La Jolla, California 92093-0608, USA. dboassa@gmail.com
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
|
