Linked Life Data

18649169

Source:http://linkedlifedata.com/resource/pubmed/id/18649169

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-7-23
pubmed:abstractText
"Atomic Force Microscope" (AFM) tips (cantilevers) carrying a pseudo-specific ligand, i.e., histidine were prepared and investigated for detection of Human Immunoglobulin-G (HIgG) in aqueous media. The AFM tips (cantilevers) were first treated with HNO(3) and silanized to create amino groups; then glutaraldehyde (GA) was bonded via these surface amino groups; and finally, histidine molecules were immobilized by reaction of the amino groups of histidine with the free aldehyde groups of GA. Optimal immobilization conditions were described. Immobilizations were observed both by optical and confocal laser scanning microscopy. Interactions between the histidine carrying AFM tips (cantilevers) and the aqueous medium containing HIgG with different concentrations were quantified by "the separation distance" measured with the AFM system as the main variable. A quite nice linear correlation between the HIgG concentration and the separation distance was measured with AFM system. Interactions were also followed by an alternative "Modified Lowry" method, in which similar behavior was observed. We were able to measure HIgG concentration in aqueous media down to 0.055 pmol/micro l (8 mg/dl) concentration with this AFM based novel immunosensor.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1532-4184
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
340-51
pubmed:dateRevised
2009-6-23
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
HIgG detection by histidine carrying AFM tips (cantilevers).
pubmed:affiliation
Baskent University, Ankara, Turkey.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't