Linked Life Data

18648921

Source:http://linkedlifedata.com/resource/pubmed/id/18648921

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-8-15
pubmed:abstractText
Thymidylate synthase (TS) is essential for de novo synthesis of dTMP and is a key enzyme involved in DNA synthesis and transcriptional regulation of organisms. Due to their biologic importance, TSs have been intensively studied. In this investigation, a thermostable TS was identified from a deep-sea thermophilic bacteriophage Geobacillus virus E2 (GVE2). It was demonstrated that GVE2-TS was highly homologous to known TSs and contained five characteristic conserved domains. The temporal analyses by Northern and Western blots revealed that the GVE2-TS was transcribed and expressed early after Geobacillus virus E2 infection, identifying it as a viral early gene. As shown by gel mobility shift assays, the recombinant GVE2-TS protein had the capacity to bind its own mRNA. Our study presented the first report on thymidylate synthase from deep-sea thermophilic bacteriophage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0920-8569
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
218-24
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Identification and characterization of a novel thymidylate synthase from deep-sea thermophilic bacteriophage Geobacillus virus E2.
pubmed:affiliation
School of Life Sciences, Xiamen University, Xiamen, 361005, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't