Linked Life Data

18646260

Source:http://linkedlifedata.com/resource/pubmed/id/18646260

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2008-7-29
pubmed:abstractText
Fast analysis in LC can be performed with sub-2 microm particles at very high pressures (up to 1000 bar) known as ultra performance LC (UPLC). With this configuration, it is possible to obtain fast and/or highly efficient separations compared to conventional LC. For the analysis of compounds without chromophores, the evaporative light scattering detector (ELSD) is an attractive alternative because of its quasi-universality, versatility, low-cost and good sensitivity. The UPLC-ELSD was investigated in terms of sensitivity and apparent efficiency, with a conventional ELSD instrument, for two types of commercially available nebulisers, using different mobile phase flow rates and column ids. Results were finally compared with the UPLC-UV configuration. Three applications with phytochemical compounds were selected to highlight the potential of this approach (i.e. the isocratic separations of artemisinin and its derivatives, of calystegines and the gradient separation of several tropane alkaloids). Depending on the used column length, baseline separations were obtained in 3-10 min, with an average apparent efficiency ranging from 7000 to 30,000 plates.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1615-9314
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2377-87
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Evaluation of the coupling between ultra performance liquid chromatography and evaporative light scattering detector for selected phytochemical applications.
pubmed:affiliation
Laboratory of Phytoanalysis, Dipartimento di Scienza e Tecnologia del Farmaco, Turin University, Turin, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies