Linked Life Data

18644311

Source:http://linkedlifedata.com/resource/pubmed/id/18644311

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2008-8-4
pubmed:abstractText
Three-dimensional cultures in a microfabricated environment provide in vivo-like conditions for cells, and have been used in a variety of applications in basic and clinical studies. In this study, the contractility of cardiomyocytes in a 3D environment using complex 3D hybrid biopolymer microcantilevers was quantified and compared with that observed in a 2D environment. By measuring the deflections of the microcantilevers with different surfaces and carrying out finite element modeling (FEM) of the focal pressures of the microcantilevers, it was found that the contractile force of high-density cardiomyocytes on 3D grooved surfaces was 65-85% higher than that of cardiomyocytes on flat surfaces. These results were supported by immunostaining, which showed alignment of the cytoskeleton and elongation of the nuclei, as well as by quantitative RT-PCR, which revealed that cells on the grooved surface had experienced sustained stimuli and tighter cell-to-cell interactions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9290
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2396-401
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Quantitative evaluation of cardiomyocyte contractility in a 3D microenvironment.
pubmed:affiliation
Nano-Bio Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Republic of Korea. jinseok@kist.re.kr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't