Linked Life Data

18641052

Source:http://linkedlifedata.com/resource/pubmed/id/18641052

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2008-9-29
pubmed:abstractText
Infiltrated macrophages (Mphi) are believed to cause pathological changes in the surrounding adipocytes through the secretion of active molecules in visceral fat. Matrix metalloproteinase (MMP)-3 is secreted from Mphi, and enhances expression of the inflammatory cytokines through the activation of toll-like receptor (TLR) 2. Visceral adipocytes express high levels of vascular endothelial growth factor (VEGF), and the degree of visceral fat accumulation is associated with the plasma VEGF concentration in obese subjects. The aim of the study is to clarify the role of MMP-3 in the enhancement of the free fatty acids (FFAs)-induced VEGF expression through TLR2 in visceral adipocytes. One mM FFAs induced VEGF mRNA and protein expression in 3T3-L1 adipocytes. The FFAs-induced VEGF expression was mostly mediated by TLR2. A high fat intake increased the VEGF mRNA expression in visceral fat and the VEGF concentration in plasma, accompanied with the increase in the plasma FFAs concentration in mice. These increases were largely inhibited in TLR2-deficient mice. The FFAs-induced VEGF expression was increased in the presence of Mphi-conditioned medium (CM) in adipocytes, and the enhancement was inhibited by a MMP-3 inhibitor or a neutralizing antibody against MMP-3. The active form of MMP-3 induced the VEGF mRNA expression, as well as TLR2, in adipocytes. The increase in the VEGF expression by MMP-3 was inhibited by the treatment with siRNA for TLR2. The enhancement of FFAs-induced TLR2 expression by Mphi-CM was inhibited by blocking of the MMP-3. The increase in the VEGF mRNA expression by Mphi-CM or MMP-3 was partially inhibited by a neutralizing antibody against TNF-alpha. These results indicate that MMP-3 in Mphi-CM enhances the FFAs-induced VEGF expression in adipocytes through the increase in the TLR2 expression. The MMP-3 secreted from the infiltrated Mphi may be a regulator of the VEGF expression in visceral adipocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1535-3702
pubmed:author
pubmed:issnType
Print
pubmed:volume
233
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1213-21
pubmed:meshHeading
pubmed-meshheading:18641052-3T3-L1 Cells, pubmed-meshheading:18641052-Adipocytes, pubmed-meshheading:18641052-Animals, pubmed-meshheading:18641052-Antibodies, pubmed-meshheading:18641052-Culture Media, Conditioned, pubmed-meshheading:18641052-Fatty Acids, Nonesterified, pubmed-meshheading:18641052-Intra-Abdominal Fat, pubmed-meshheading:18641052-Macrophages, pubmed-meshheading:18641052-Matrix Metalloproteinase 3, pubmed-meshheading:18641052-Mice, pubmed-meshheading:18641052-Mice, Inbred C57BL, pubmed-meshheading:18641052-Mice, Knockout, pubmed-meshheading:18641052-RNA, Messenger, pubmed-meshheading:18641052-RNA, Small Interfering, pubmed-meshheading:18641052-Toll-Like Receptor 2, pubmed-meshheading:18641052-Tumor Necrosis Factor-alpha, pubmed-meshheading:18641052-Vascular Endothelial Growth Factor A
pubmed:year
2008
pubmed:articleTitle
Matrix metalloproteinase-3 enhances the free fatty acids-induced VEGF expression in adipocytes through toll-like receptor 2.
pubmed:affiliation
Department of Genome Research and Clinical Application, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't