Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7-8
pubmed:dateCreated
1977-1-29
pubmed:abstractText
Cell separation techniques normally used to obtain subpopulations of lymphocytes were shown, under certain conditions, to render the cells cytotoxic towards a number of target-cells including autologous lymphocytes. To cause cytotoxicity, it was necessary to pass cells through glass wool and/or nylon wool columns equilibrated with media containing fresh plasma or serum. Cells lost activation upon overnight in vitro culture or treatment with trypsin. In addition to direct cell cytotoxicity, cells released heat labile cytotoxic factors and antibody during the separation procedures. The implications of the results for the interpretation of cell separation studies designed to attribute immunological effects to one or another cell type were discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0090-0877
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
637-48
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Lymphocyte activation by cell separation procedures.
pubmed:publicationType
Journal Article