Linked Life Data

18638251

Source:http://linkedlifedata.com/resource/pubmed/id/18638251

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-7-21
pubmed:abstractText
Nuclear DNA was extracted from human telogen hairs from 60 individuals. Six to nine hairs from each individual were individually extracted. The amount of DNA recovered from each individual varied greatly, and most samples yielded a quantity of 550 pg or less per hair. A selective extraction buffer was used to remove epithelial cell DNA and the amount of exogenous DNA was determined. DNA was also quantified by real time PCR using three different sized amplicons targeting an Alu sequence. The results were used to determine the state of degradation of the extracted DNA. Different quantities of sample (<100 pg, 100-500 pg, >500 pg) were amplified with the Miniplex kits to determine the minimum DNA template required for successful amplification. DNA recovered from hair showed degradation; however, partial profiles were obtained for those samples containing at least 60 pg using MiniSTRs.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1556-4029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
853-7
pubmed:dateRevised
2009-3-3
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Evaluation and quantification of nuclear DNA from human telogen hairs.
pubmed:affiliation
Department of Chemistry and Biochemistry, Florida International University, University Park, 11200 SW 8th Street, Miami, FL 33199, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.