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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2008-12-17
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pubmed:abstractText |
The role of natural killer (NK) cells in controlling hepatitis C virus (HCV) infection and replication has not been fully delineated. We examined NK cell-mediated noncytolytic effect on full cycle HCV infection of human hepatocytes. Human hepatocytes (Huh7.5.1 cells) co-cultured with NK cells or treated with supernatants (SN) from NK cells cultures had significantly lower levels of HCV RNA and protein than control cells. This NK cell-mediated anti-HCV activity could be largely abolished by antibody to interferon-gamma (IFN-gamma). The investigation of the mechanisms for NK cell-mediated anti-HCV activity showed that NK SN-treated hepatocytes expressed higher levels of IFN-alpha/beta than the control cells. NK SN also enhanced IFN regulatory factor-3 and 7 expression in the hepatocytes. In addition, NK SN enhanced the expression of signal transducer and activator of transcription 1 and 2, the nuclear factors that are essential for the activation of IFN-mediated antiviral pathways. These data provide direct evidence at cellular and molecular levels that NK cells have a key role in suppressing HCV infection of and replication in human hepatocytes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-10875919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-11050046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-11092253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-11256746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-11690712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-11781363,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-11870386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-11879700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-12217606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-12453636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-12691460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-12692549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-12702807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-12774012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-12937158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-14525967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-15339939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-15922665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-15939869,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-15951748,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-9039782,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18637071-9877175
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1365-2893
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
855-64
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pubmed:dateRevised |
2010-12-3
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pubmed:meshHeading |
pubmed-meshheading:18637071-Adult,
pubmed-meshheading:18637071-Cells, Cultured,
pubmed-meshheading:18637071-Coculture Techniques,
pubmed-meshheading:18637071-Down-Regulation,
pubmed-meshheading:18637071-Female,
pubmed-meshheading:18637071-Gene Expression,
pubmed-meshheading:18637071-Hepacivirus,
pubmed-meshheading:18637071-Hepatitis C,
pubmed-meshheading:18637071-Hepatocytes,
pubmed-meshheading:18637071-Humans,
pubmed-meshheading:18637071-Interferons,
pubmed-meshheading:18637071-Killer Cells, Natural,
pubmed-meshheading:18637071-Male,
pubmed-meshheading:18637071-Signal Transduction,
pubmed-meshheading:18637071-Virus Replication
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pubmed:year |
2008
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pubmed:articleTitle |
Natural killer cells suppress full cycle HCV infection of human hepatocytes.
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pubmed:affiliation |
Division of Allergy and Immunology, Joseph Stokes Jr. Research Institute at The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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