Linked Life Data

18635114

Source:http://linkedlifedata.com/resource/pubmed/id/18635114

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-7-18
pubmed:abstractText
Distant antigenic relationships between Australian strains of tomato aspermy virus (TAV) and cucumber mosaic virus (CMV) were investigated by three serological methods: radial double immunodiffusion in agar, enzyme-linked immunosorbent assays (ELISA), and immune-electron microscopy (IEM) using the antibody decoration technique. Based on the work with TAV and CMV presented in this paper, it appears that the ELISA method is especially suitable for studying antigenic relationships among viruses. For distinguishing closely related viruses, heterologous antibodies were used for both coating the ELISA plates and for conjugating with enzyme. On the other hand, for establishing distant antigenic relationships, heterologous and homologous antibodies were used for coating and coupling to enzyme, respectively. Immunodiffusion tests were less efficient than ELISA in distinguishing minor antigenic differences between strains of CMV and also, and distant relationship between TAV and CMV was not as clearly detected. Decoration of the virus particles with antibodies in IEM was the least satisfactory method for investigating antigenic relationships among the viruses.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jan
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
318-26
pubmed:year
1982
pubmed:articleTitle
Comparative studies on tomato aspermy and cucumber mosaic viruses. VII. Serological relationships reinvestigated.
pubmed:affiliation
Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, South Australia, Australia.
pubmed:publicationType
Journal Article