Linked Life Data

18634860

Source:http://linkedlifedata.com/resource/pubmed/id/18634860

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-8-25
pubmed:abstractText
Polycyclic aromatic hydrocarbons (PAHs) are an important group of environmental pollutants, known for their mutagenic and carcinogenic activities. Many PAHs are aryl hydrocarbon receptor (AhR) ligands and several recent studies have suggested that PAHs or their metabolites may activate estrogen receptors (ER). The present study investigated possible estrogenic/antiestrogenic effects of abundant environmental contaminants benzo[a]pyrene (BaP), benz[a]anthracene (BaA), fluoranthene (Fla) and benzo[k]fluoranthene (BkF) in vivo, using the immature rat uterotrophic assay. The present results suggest that BaA, BaP and Fla behaved as estrogen-like compounds in immature Wistar rats, when applied for 3 consecutive days at 10mg/kg/day, as documented by a significant increase of uterine weight and hypertrophy of luminal epithelium. These effects were likely to be mediated by ERalpha, a major subtype of ER present in uterus, as they were inhibited by treatment with ER antagonist ICI 182,780. BaA, the most potent of studied PAHs, induced a significant estrogenic effect within a concentration range 0.1-50mg/kg/day; however, it did not reach the maximum level induced by reference estrogens. The proposed antiestrogenicity of the potent AhR agonist BkF was not confirmed in the present in vivo study; the exposure to BkF did not significantly affect the uterine weight, although a weak suppression of ERalpha immunostaining was observed in luminal and glandular epithelium, possibly related to its AhR-mediated activity. The PAHs under study did not induce marked genotoxic damage in uterine tissues, as documented by the lack of Ser-15-phoshorylated p53 protein staining. With the exception of Fla, all three remaining compounds increased CYP1-dependent monooxygenation activities in liver at the doses used, suggesting that the potential tissue-specific antiestrogenic effects of PAHs mediated by metabolization of 17beta-estradiol also cannot be excluded. Taken together, these environmentally relevant PAHs induced estrogenic effects in vivo, which might affect their toxic impact and carcinogenicity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0378-4274
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
180
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
212-21
pubmed:meshHeading
pubmed-meshheading:18634860-Animals, pubmed-meshheading:18634860-Cytochrome P-450 CYP1A1, pubmed-meshheading:18634860-Endocrine Disruptors, pubmed-meshheading:18634860-Environmental Pollutants, pubmed-meshheading:18634860-Epithelium, pubmed-meshheading:18634860-Estradiol, pubmed-meshheading:18634860-Estrogen Receptor alpha, pubmed-meshheading:18634860-Estrogens, pubmed-meshheading:18634860-Female, pubmed-meshheading:18634860-Hydroxylation, pubmed-meshheading:18634860-Immunohistochemistry, pubmed-meshheading:18634860-Microsomes, Liver, pubmed-meshheading:18634860-Organ Size, pubmed-meshheading:18634860-Ovary, pubmed-meshheading:18634860-Phosphorylation, pubmed-meshheading:18634860-Polycyclic Hydrocarbons, Aromatic, pubmed-meshheading:18634860-Rats, pubmed-meshheading:18634860-Rats, Wistar, pubmed-meshheading:18634860-Tumor Suppressor Protein p53, pubmed-meshheading:18634860-Uterus
pubmed:year
2008
pubmed:articleTitle
Estrogenic activity of environmental polycyclic aromatic hydrocarbons in uterus of immature Wistar rats.
pubmed:affiliation
Veterinary Research Institute, Brno 62132, Czech Republic.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't