| pubmed-article:18633188 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18633188 | lifeskim:mentions | umls-concept:C0231337 | lld:lifeskim |
| pubmed-article:18633188 | lifeskim:mentions | umls-concept:C0038250 | lld:lifeskim |
| pubmed-article:18633188 | lifeskim:mentions | umls-concept:C0014257 | lld:lifeskim |
| pubmed-article:18633188 | lifeskim:mentions | umls-concept:C0003018 | lld:lifeskim |
| pubmed-article:18633188 | lifeskim:mentions | umls-concept:C0071097 | lld:lifeskim |
| pubmed-article:18633188 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:18633188 | pubmed:dateCreated | 2008-7-17 | lld:pubmed |
| pubmed-article:18633188 | pubmed:abstractText | We investigated whether a peroxisome proliferator-activated receptor (PPAR) agonist would effect the angiotensin II (Ang II)-induced senescence of endothelial progenitor cells (EPCs). EPCs were isolated from peripheral blood and characterized. Both reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to assess gp91phox expression and angiotensin type 1 receptor (AT1R) levels. Immunofluorescence of nitrotyrosine provided evidence of peroxynitrite formation. Our data indicate that Ang II increased the expression of gp91phox mRNA, which was significantly diminished by pioglitazone, a PPARgamma agonist. Western blotting revealed that Ang II stimulated an increase in the gp91phox protein, whereas co-treatment with pioglitazone significantly reduced this increase. In addition, pioglitazone also inhibited Ang II-induced peroxynitrite formation. Interestingly, pioglitazone decreased the expressions of AT1R mRNA and protein. The exposure of cultured EPCs to Ang II (100 nmol/L) significantly accelerated the rate of senescence compared to that of the control cells during 14 d in culture, as determined by acidic beta-galactosidase staining. Ang II-induced EPC senescence was significantly inhibited by co-treatment with pioglitazone. Because cellular senescence is critically influenced by telomerase, which elongates telomeres, we also measured telomerase activity by means of PCR-ELISA-based assay. The results showed that Ang II significantly diminished telomerase activity, and this effect was significantly abolished by co-treatment with pioglitazone. In conclusion, pioglitazone inhibited Ang II-induced senescence of EPCs via down-regulation of the expression of AT1R. | lld:pubmed |
| pubmed-article:18633188 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18633188 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18633188 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18633188 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:18633188 | pubmed:issn | 0916-9636 | lld:pubmed |
| pubmed-article:18633188 | pubmed:author | pubmed-author:AkasakaTakash... | lld:pubmed |
| pubmed-article:18633188 | pubmed:author | pubmed-author:ImanishiToshi... | lld:pubmed |
| pubmed-article:18633188 | pubmed:author | pubmed-author:KobayashiKats... | lld:pubmed |
| pubmed-article:18633188 | pubmed:author | pubmed-author:KuroiAkioA | lld:pubmed |
| pubmed-article:18633188 | pubmed:author | pubmed-author:IkejimaHideyu... | lld:pubmed |
| pubmed-article:18633188 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18633188 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:18633188 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18633188 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18633188 | pubmed:pagination | 757-65 | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:meshHeading | pubmed-meshheading:18633188... | lld:pubmed |
| pubmed-article:18633188 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18633188 | pubmed:articleTitle | Pioglitazone inhibits angiotensin II-induced senescence of endothelial progenitor cell. | lld:pubmed |
| pubmed-article:18633188 | pubmed:affiliation | Department of Cardiovascular Medicine, Wakayama Medical University, Wakayama, Japan. t-imani@wakayama-med.ac.jp | lld:pubmed |
| pubmed-article:18633188 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18633188 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18633188 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18633188 | lld:pubmed |
