Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-7-17
pubmed:abstractText
We investigated whether a peroxisome proliferator-activated receptor (PPAR) agonist would effect the angiotensin II (Ang II)-induced senescence of endothelial progenitor cells (EPCs). EPCs were isolated from peripheral blood and characterized. Both reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to assess gp91phox expression and angiotensin type 1 receptor (AT1R) levels. Immunofluorescence of nitrotyrosine provided evidence of peroxynitrite formation. Our data indicate that Ang II increased the expression of gp91phox mRNA, which was significantly diminished by pioglitazone, a PPARgamma agonist. Western blotting revealed that Ang II stimulated an increase in the gp91phox protein, whereas co-treatment with pioglitazone significantly reduced this increase. In addition, pioglitazone also inhibited Ang II-induced peroxynitrite formation. Interestingly, pioglitazone decreased the expressions of AT1R mRNA and protein. The exposure of cultured EPCs to Ang II (100 nmol/L) significantly accelerated the rate of senescence compared to that of the control cells during 14 d in culture, as determined by acidic beta-galactosidase staining. Ang II-induced EPC senescence was significantly inhibited by co-treatment with pioglitazone. Because cellular senescence is critically influenced by telomerase, which elongates telomeres, we also measured telomerase activity by means of PCR-ELISA-based assay. The results showed that Ang II significantly diminished telomerase activity, and this effect was significantly abolished by co-treatment with pioglitazone. In conclusion, pioglitazone inhibited Ang II-induced senescence of EPCs via down-regulation of the expression of AT1R.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II, http://linkedlifedata.com/resource/pubmed/chemical/CYBB protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Hypoglycemic Agents, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/NADPH Oxidase, http://linkedlifedata.com/resource/pubmed/chemical/Peroxynitrous Acid, http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 1, http://linkedlifedata.com/resource/pubmed/chemical/Superoxides, http://linkedlifedata.com/resource/pubmed/chemical/Telomerase, http://linkedlifedata.com/resource/pubmed/chemical/Thiazolidinediones, http://linkedlifedata.com/resource/pubmed/chemical/Vasoconstrictor Agents, http://linkedlifedata.com/resource/pubmed/chemical/pioglitazone
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0916-9636
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
757-65
pubmed:meshHeading
pubmed-meshheading:18633188-Angiotensin II, pubmed-meshheading:18633188-Cell Aging, pubmed-meshheading:18633188-Cells, Cultured, pubmed-meshheading:18633188-Drug Interactions, pubmed-meshheading:18633188-Gene Expression, pubmed-meshheading:18633188-Hematopoietic Stem Cells, pubmed-meshheading:18633188-Humans, pubmed-meshheading:18633188-Hypertension, pubmed-meshheading:18633188-Hypoglycemic Agents, pubmed-meshheading:18633188-Membrane Glycoproteins, pubmed-meshheading:18633188-NADPH Oxidase, pubmed-meshheading:18633188-Oxidative Stress, pubmed-meshheading:18633188-Peroxynitrous Acid, pubmed-meshheading:18633188-Receptor, Angiotensin, Type 1, pubmed-meshheading:18633188-Superoxides, pubmed-meshheading:18633188-Telomerase, pubmed-meshheading:18633188-Thiazolidinediones, pubmed-meshheading:18633188-Vasoconstrictor Agents
pubmed:year
2008
pubmed:articleTitle
Pioglitazone inhibits angiotensin II-induced senescence of endothelial progenitor cell.
pubmed:affiliation
Department of Cardiovascular Medicine, Wakayama Medical University, Wakayama, Japan. t-imani@wakayama-med.ac.jp
pubmed:publicationType
Journal Article