Linked Life Data

18632802

Source:http://linkedlifedata.com/resource/pubmed/id/18632802

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-10-10
pubmed:abstractText
Adjustment of Na+ balance in extracellular fluids is achieved by regulated Na+ transport involving the amiloride-sensitive epithelial Na+ channel (ENaC) in the distal nephron. In this context, ENaC is controlled by a number of hormones, including vasopressin, which promotes rapid translocation of water and Na+ channels to the plasma membrane and long-term effects on transcription of vasopressin-induced and -reduced transcripts. We have identified a mRNA encoding the deubiquitylating enzyme ubiquitin-specific protease 10 (Usp10), whose expression is increased by vasopressin at both the mRNA and the protein level. Coexpression of Usp10 in ENaC-transfected HEK-293 cells causes a more than fivefold increase in amiloride-sensitive Na+ currents, as measured by whole cell patch clamping. This is accompanied by a three- to fourfold increase in surface expression of alpha- and gamma-ENaC, as shown by cell surface biotinylation experiments. Although ENaC is well known to be regulated by its direct ubiquitylation, Usp10 does not affect the ubiquitylation level of ENaC, suggesting an indirect effect. A two-hybrid screen identified sorting nexin 3 (SNX3) as a novel substrate of Usp10. We show that it is a ubiquitylated protein that is degraded by the proteasome; interaction with Usp10 leads to its deubiquitylation and stabilization. When coexpressed with ENaC, SNX3 increases the channel's cell surface expression, similarly to Usp10. In mCCD(cl1) cells, vasopressin increases SNX3 protein but not mRNA, supporting the idea that the vasopressin-induced Usp10 deubiquitylates and stabilizes endogenous SNX3 and consequently promotes cell surface expression of ENaC.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1931-857X
pubmed:author
pubmed:issnType
Print
pubmed:volume
295
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F889-900
pubmed:dateRevised
2011-4-28
pubmed:meshHeading
pubmed-meshheading:18632802-Animals, pubmed-meshheading:18632802-Antidiuretic Agents, pubmed-meshheading:18632802-Carrier Proteins, pubmed-meshheading:18632802-Cell Line, pubmed-meshheading:18632802-Cell Membrane, pubmed-meshheading:18632802-Epithelial Sodium Channel, pubmed-meshheading:18632802-Humans, pubmed-meshheading:18632802-Kidney, pubmed-meshheading:18632802-Membrane Potentials, pubmed-meshheading:18632802-Mice, pubmed-meshheading:18632802-RNA, Messenger, pubmed-meshheading:18632802-Rats, pubmed-meshheading:18632802-Sodium, pubmed-meshheading:18632802-Sorting Nexins, pubmed-meshheading:18632802-Transfection, pubmed-meshheading:18632802-Two-Hybrid System Techniques, pubmed-meshheading:18632802-Ubiquitin, pubmed-meshheading:18632802-Ubiquitin Thiolesterase, pubmed-meshheading:18632802-Vasopressins, pubmed-meshheading:18632802-Vesicular Transport Proteins
pubmed:year
2008
pubmed:articleTitle
Vasopressin-inducible ubiquitin-specific protease 10 increases ENaC cell surface expression by deubiquitylating and stabilizing sorting nexin 3.
pubmed:affiliation
Department of Pharmacology and Toxicology, University of Lausanne, Rue du Bugnon 27, CH-1005 Lausanne, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't