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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2008-9-5
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pubmed:abstractText |
Mitochondria from plants, yeast, and animals each contain at least one peroxiredoxin (Prx) that is involved in peroxide detoxification and redox signalling. The supramolecular dynamics of atypical type II Prx targeted to the mitochondrion was addressed in pea. Microcalorimetric (ITC) titrations identified an extremely high-affinity binding between the mitochondrial PsPrxIIF and Trx-o with a K(D) of 126+/-14 pM. Binding was driven by a favourable enthalpy change (DeltaH= -60.6 kcal mol(-1)) which was counterbalanced by unfavourable entropy changes (TDeltaS= -47.1 kcal mol(-1)). This is consistent with the occurrence of large conformational changes during binding which was abolished upon site-directed mutaganesis of the catalytic C59S and C84S. The redox-dependent interaction was confirmed by gel filtration of mitochondrial extracts and co-immunoprecipitation from extracts. The heterocomplex of PsPrxIIF and Trx-o reduced peroxide substrates more efficiently than free PsPrxIIF suggesting that Trx-o serves as an efficient and specific electron donor to PsPrxIIF in vivo. Other Trx-s tested by ITC analysis failed to interact with PsPrxIIF indicating a specific recognition of PsPrxIIF by Trx-o. PsPrxIIF exists primarily as a dimer or a hexamer depending on the redox state. In addition to the well-characterized oligomerization of classical 2-Cys Prx the results also show that atypical Prx undergo large structural reorganization with implications for protein-protein interaction and function.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-10198100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-10213627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-10679306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-10956001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-11836238,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-12478295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-12529539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-12913160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-15333640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-15632145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-15697201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-15848167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-15886207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-15941719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-16507087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-16906481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-17095008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-17586656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-17707404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-18553980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-7599130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-8496166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18632730-8554508
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1460-2431
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3259-69
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Hexameric oligomerization of mitochondrial peroxiredoxin PrxIIF and formation of an ultrahigh affinity complex with its electron donor thioredoxin Trx-o.
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pubmed:affiliation |
Biochemistry and Physiology of Plants, W5-134, Bielefeld University, D-33501 Bielefeld, Germany. sergio.barranco@uni-bielefeld.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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