Linked Life Data

18629659

Source:http://linkedlifedata.com/resource/pubmed/id/18629659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-7-16
pubmed:abstractText
During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
422
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-49
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
FISH for mapping single copy genes.
pubmed:affiliation
Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't